Ramos F, Santos C, Silva A, da Silveira M I
Laboratório de Bromatologia, Nutrição e Hidrologia, Faculdade de Farmácia, Universidade de Coimbra, Portugal.
J Chromatogr B Biomed Sci Appl. 1998 Sep 25;716(1-2):366-70. doi: 10.1016/s0378-4347(98)00292-8.
A derivatization procedure for confirmatory residue analysis of beta2-agonists is described. Methyl (MBA) and butyl (BBA) boronic acids are simultaneously used for the derivatization of tulobuterol, mabuterol, mapenterol, salbutamol, clenproperol, clenbuterol, clenpenterol and bromobuterol by GC-MS determination. A temperature of 55 degrees C during 60 min was selected as optimal temperature-time condition for simultaneous MBA and BBA beta2-agonists derivatization. It was also observed that stability of boronic derivatives was maintained at -20 degrees C over a period of four days. The proposed methodology was tested in urine and it could be applied for confirmatory residue analysis of clenbuterol-like compounds.
本文描述了一种用于β2-激动剂残留确证分析的衍生化方法。通过气相色谱-质谱联用（GC-MS）测定，同时使用甲基硼酸（MBA）和丁基硼酸（BBA）对妥布特罗、马布特罗、马喷特罗、沙丁胺醇、氯丙那林、克伦特罗、氯戊丁胺和溴布特罗进行衍生化。选择55℃反应60分钟作为MBA和BBA同时对β2-激动剂进行衍生化的最佳温度-时间条件。还观察到硼酸衍生物在-20℃下可保持四天的稳定性。所提出的方法在尿液中进行了测试，可用于克伦特罗类化合物的确证残留分析。
