Fernández-Fernández M R, Martínez-Torrecuadrada J L, Casal J I, García J A
Centro Nacional de Biotecnología (CNB-CSIC), Campus de la Universidad Autónoma de Madrid, Spain.
FEBS Lett. 1998 May 8;427(2):229-35. doi: 10.1016/s0014-5793(98)00429-3.
The development of an antigen presentation system based on the plum pox potyvirus (PPV) is here described. The amino-terminal part of PPV capsid protein was chosen as the site for expression of foreign antigenic peptides. Modifications in this site were engineered to avoid the capability of natural transmission by aphids of this PPV vector. As a first practical attempt, different forms of an antigenic peptide (single and tandem repetition) from the VP2 capsid protein of canine parvovirus (CPV) were expressed. Both chimeras are able to infect Nicotiana clevelandii plants with similar characteristics to wild-type virus and remain genetically stable after several plant passages. The antigenicity of purified chimeric virions was demonstrated, proving the suitability of this system for diagnostic purposes. Moreover, mice and rabbits immunized with chimeric virions developed CPV-specific antibodies, which showed neutralizing activity.
本文描述了一种基于李痘病毒（PPV）的抗原呈递系统的开发。选择PPV衣壳蛋白的氨基末端部分作为外源抗原肽的表达位点。对该位点进行了改造，以避免这种PPV载体通过蚜虫自然传播的能力。作为首次实际尝试，表达了来自犬细小病毒（CPV）VP2衣壳蛋白的不同形式的抗原肽（单拷贝和串联重复）。两种嵌合体都能够感染克利夫兰烟草植株，其特性与野生型病毒相似，并且在经过几次植物传代后仍保持遗传稳定性。纯化的嵌合病毒粒子的抗原性得到了证实，证明了该系统适用于诊断目的。此外，用嵌合病毒粒子免疫的小鼠和兔子产生了CPV特异性抗体，这些抗体具有中和活性。
