Slyshenkov V S, Omelyanchik S N, Moiseenok A G, Trebukhina R V, Wojtczak L
Institute of Biochemistry, Academy of Sciences of Belarus, Grodno.
Free Radic Biol Med. 1998 Apr;24(6):894-9. doi: 10.1016/s0891-5849(97)00378-x.
Rats were exposed to gamma radiation from a 60Co source, receiving 0.25 Gy at weekly intervals. During 2 d before each irradiation, the animals received daily intragastric doses of 26 mg pantothenol or 15 mg beta-carotene per kg body weight. One hour after the third irradiation session, the animals were killed and their livers were analyzed. In animals not supplied with pantothenol, the irradiation resulted in a significant decrease of total liver lipids and a 50% decrease in phospholipids. Liver cholesterol was decreased by about 20%. Irradiation produced lipid peroxidation as expressed by doubling of the amounts of conjugated dienes and ketone dienes and of thiobarbituric acid reactive compounds. The amount of CoA in liver was decreased by 24% and that of reduced glutathione by 40%. The NAD+/NADH ratio was increased by 60% and the activity of NADP-dependent malate dehydrogenase (decarboxylating) was decreased by 26%. The amount of pantothenic acid and its derivatives (expressed as pantolactone-generating compounds) in blood decreased by about 80%. In rats to which pantothenol was administered, the content of pantothenic acid in blood was tripled compared to nonirradiated (control) rats, and all the biochemical parameters measured in liver were the same as in nonirradiated animals.
将大鼠暴露于来自60Co源的γ辐射下，每周接受0.25 Gy的辐射。在每次辐射前的2天里，动物每天接受每千克体重26毫克泛醇或15毫克β-胡萝卜素的胃内给药。在第三次辐射 session 后1小时，处死动物并分析其肝脏。在未供应泛醇的动物中，辐射导致肝脏总脂质显著减少，磷脂减少50%。肝脏胆固醇减少约20%。辐射产生脂质过氧化，表现为共轭二烯和酮二烯以及硫代巴比妥酸反应性化合物的量增加一倍。肝脏中辅酶A的量减少24%，还原型谷胱甘肽的量减少40%。NAD+/NADH比值增加60%，NADP依赖性苹果酸脱氢酶（脱羧）的活性降低26%。血液中泛酸及其衍生物（以产生泛内酯的化合物表示）的量减少约80%。在给予泛醇的大鼠中，与未辐照（对照）大鼠相比，血液中泛酸的含量增加了两倍，并且在肝脏中测量的所有生化参数与未辐照动物相同。
