Molinari G, Chhatwal G S
Division of Microbiology, Technical University/GBF-National Research Centre for Biotechnology, Braunschweig, Germany.
J Infect Dis. 1998 Jun;177(6):1600-7. doi: 10.1086/515310.
The invasiveness of 96 group A streptococci (GAS) isolates (56 from throat or skin and 40 from blood) were analyzed. GAS invasion strongly correlated with the source of the isolates, whereas no correlation was observed with the Vir type. Isolates from throat or skin exhibited the highest invasion efficiency (57% were between 0.1% and 10%). In contrast, 77.5% of the blood isolates were noninvasive (efficiency <0.01%) and only 7.5% exhibited rates comparable to those of throat or skin isolates (>0.1%). Immunofluorescence studies of 34 selected isolates showed that attachment and invasion are strain-related. Although isolates with high invasiveness usually exhibit high attachment, isolates that showed high attachment and no invasion or poor attachment and efficient internalization were identified. The ability of GAS to invade and survive within eukaryotic cells may provide bacteria a sure niche, in which they are protected against host defense mechanisms or antimicrobial agents favoring their local persistence.
对96株A组链球菌（GAS）分离株（56株来自咽喉或皮肤，40株来自血液）的侵袭性进行了分析。GAS的侵袭与分离株的来源密切相关，而与Vir型无关。来自咽喉或皮肤的分离株表现出最高的侵袭效率（57%介于0.1%至10%之间）。相比之下，77.5%的血液分离株无侵袭性（效率<0.01%），只有7.5%的侵袭率与咽喉或皮肤分离株相当（>0.1%）。对34株选定分离株的免疫荧光研究表明，黏附和侵袭与菌株相关。尽管侵袭性高的分离株通常表现出高黏附性，但也鉴定出了黏附性高但无侵袭性或黏附性差但内化效率高的分离株。GAS在真核细胞内侵袭和存活的能力可能为细菌提供了一个稳定的生态位，在其中它们受到保护，免受宿主防御机制或抗菌剂的影响，有利于它们在局部持续存在。
