Shearer M H, Timanus D K, Benton P A, Lee D R, Kennedy R C
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
J Infect Dis. 1998 Jun;177(6):1727-9. doi: 10.1086/517432.
A murine monoclonal antibody (MAb) with human CD4 specificity was tested for the ability to inhibit primary human immunodeficiency virus type 1 (HIV-1) isolates clades A through E. Human peripheral blood mononuclear cells (PBMC) were used as target cells for infectivity. The HIV-1 primary isolates were examined for the capacity to infect PBMC targets in the presence or absence of the anti-CD4 MAb, designated P1. P1 broadly inhibited clade A, C, D, and E isolates, based on a reduction of HIV-1 p24 antigen concentrations compared with untreated controls. Little to no virus-inhibiting activity was observed with a primary HIV-1 clade B isolate, designated BZ167. Additionally, a second primary clade B isolate was efficiently inhibited from infecting PBMC targets by P1. The data indicate that P1 exhibits group-specific inhibiting activity against non-clade B primary HIV-1 isolates in vitro.
一种具有人CD4特异性的鼠单克隆抗体（MAb）被测试了抑制A至E亚型的1型人类免疫缺陷病毒（HIV-1）原始分离株的能力。人外周血单核细胞（PBMC）被用作感染性的靶细胞。在存在或不存在名为P1的抗CD4单克隆抗体的情况下，检测HIV-1原始分离株感染PBMC靶细胞的能力。与未处理的对照相比，基于HIV-1 p24抗原浓度的降低，P1广泛抑制A、C、D和E亚型分离株。对于名为BZ167的HIV-1 B亚型原始分离株，几乎未观察到病毒抑制活性。此外，P1有效地抑制了另一种B亚型原始分离株感染PBMC靶细胞。数据表明，P1在体外对非B亚型HIV-1原始分离株表现出组特异性抑制活性。
