Nielsen S D, Afzelius P, Dam-Larsen S, Nielsen C, Nielsen J O, Mathiesen L, Hansen J E
Department of Infectious Diseases, Hvidovre Hospital, Denmark.
J Infect Dis. 1998 Jun;177(6):1733-6. doi: 10.1086/517434.
The potential of granulocyte colony-stimulating factor (G-CSF) to mobilize CD4 cells and/or CD34 cells for use in gene therapy or to support antiretroviral therapy was examined. Ten human immunodeficiency virus-infected patients were treated with G-CSF (300 microg/day) for 5 days. Numbers of CD4 and CD34 cells were measured. To examine the numbers of naive and memory type CD4 cells, CD4 cell coexpression of CD45RA and CD45RO was measured. Functionality of mobilized CD4 cells was examined by use of the proliferation assay and interleukin-2 ELISA. The number of CD34 cells increased from 1.50 to 20.01/microL (P < .002). The CD4 cell count increased from 236 to 452/microL (P < .002). The CD45RA/CD45RO ratio increased from 0.50 to 0.57 (P < .03). Mobilized CD4 cells were functionally intact. In conclusion, G-CSF induced increases in numbers of CD34 cells and CD4 cells in HIV-infected patients. Furthermore, the fraction of naive CD4 cells increased. These findings have implications for the design of immunotherapy or gene therapy protocols.
研究了粒细胞集落刺激因子（G-CSF）动员CD4细胞和/或CD34细胞用于基因治疗或支持抗逆转录病毒治疗的潜力。10例人类免疫缺陷病毒感染患者接受G-CSF（300微克/天）治疗5天。测量CD4和CD34细胞数量。为检测初始型和记忆型CD4细胞数量，测量CD4细胞CD45RA和CD45RO的共表达情况。通过增殖试验和白细胞介素-2酶联免疫吸附测定法检测动员的CD4细胞的功能。CD34细胞数量从1.50/微升增至20.01/微升（P<.002）。CD4细胞计数从236/微升增至452/微升（P<.002）。CD45RA/CD45RO比值从0.50增至0.57（P<.03）。动员的CD4细胞功能完好。总之，G-CSF可使HIV感染患者的CD34细胞和CD4细胞数量增加。此外，初始型CD4细胞比例增加。这些发现对免疫治疗或基因治疗方案的设计具有启示意义。
