Woodside B, Abizaid A, Jafferali S
Center for Studies in Behavioral Neurobiology, Department of Psychology, Concordia University, Montreal, Quebec, Canada H4B 1R6.
Am J Physiol. 1998 Jun;274(6):R1653-8. doi: 10.1152/ajpregu.1998.274.6.R1653.
The goals of these experiments were to determine whether lactational anestrus would be prolonged by a 48-h fast at days 13 and 14 postpartum (pp) and, if so, to determine whether this effect could be reversed by treatment with the Ob protein leptin. We found that food deprivation on days 13 and 14 pp prolonged lactational infertility by 7 days and that the nutritional experience of both the dam and her litter contributed to this effect. Leptin administration (2.5 mg . kg-1 . day-1) during food deprivation was sufficient to reduce the length of lactational infertility compared with vehicle-treated food-deprived rats (P < 0.05). Similar leptin treatment in ad libitum-fed animals reduced food intake (P < 0.05) and litter growth (P < 0.05) but had no statistically significant effect on maternal weight gain or length of lactational infertility. Food-deprived lactating animals had lower circulating leptin levels than ad libitum-fed lactating animals on day 15 pp (P < 0.05), as determined by RIA. Levels in nonlactating rats were higher than in either lactating group (P < 0.05).
这些实验的目的是确定产后（pp）第13天和第14天禁食48小时是否会延长哺乳期发情间期，如果会的话，确定用Ob蛋白瘦素治疗是否可以逆转这种效应。我们发现，产后第13天和第14天禁食会使哺乳期不育期延长7天，并且母鼠及其幼崽的营养经历都促成了这种效应。与用赋形剂处理的禁食大鼠相比，禁食期间给予瘦素（2.5毫克·千克-1·天-1）足以缩短哺乳期不育期的时长（P<0.05）。对自由采食的动物进行类似的瘦素处理会减少食物摄入量（P<0.05）和幼崽生长量（P<0.05），但对母鼠体重增加或哺乳期不育期时长没有统计学上的显著影响。通过放射免疫分析测定，产后第15天，禁食的泌乳动物的循环瘦素水平低于自由采食的泌乳动物（P<0.05）。非泌乳大鼠的瘦素水平高于任何一个泌乳组（P<0.05）。
