Coutinho S D, de Souza T, Paula C R
Microbiology Department, Biomedical Science Institute, Universidade de São Paulo, Brazil.
Mycopathologia. 1997;139(3):129-35. doi: 10.1023/a:1006848114809.
The protein fractions of 30 Malassezia pachydermatis isolates from dogs (15 with otitis and 15 with dermatitis) were investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The electrophoresis runs resulted in bands ranging from 15.0-145.7 kDa in the ear canal secretion isolates. The values for the skin isolates varied between 16.0-141.1 kDa. The presence of protein bands occurred most frequently within the 30-40 kDa bands (66.7%), 40-50 kDa (70.0%), 50-60 kDa (73.3%) and 80-90 kDa (83.3%). No statistical differences were determined, considering the behavior of M. pachydermatis isolates from ear and skin in each band of molecular weight values, nor in the number of bands obtained.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对30株来自犬的厚皮马拉色菌分离株（15株患有中耳炎，15株患有皮炎）的蛋白质组分进行了研究。耳道分泌物分离株的电泳结果显示，条带范围在15.0 - 145.7 kDa之间。皮肤分离株的值在16.0 - 141.1 kDa之间变化。蛋白质条带最常出现在30 - 40 kDa条带（66.7%）、40 - 50 kDa（70.0%）、50 - 60 kDa（73.3%）和80 - 90 kDa（83.3%）范围内。考虑到来自耳部和皮肤的厚皮马拉色菌分离株在每个分子量值条带中的表现以及获得的条带数量，未确定统计学差异。
