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枯草芽孢杆菌的早期阻断产孢突变体被温和噬菌体溶源化的频率较低。

Early-blocked asporogenous mutants of Bacillus subtilis are lysogenized at reduced frequency by temperate bacteriophages.

作者信息

Ikeuchi T, Kurahashi K

出版信息

J Bacteriol. 1978 May;134(2):440-5. doi: 10.1128/jb.134.2.440-445.1978.

Abstract

The establishment of lysogeny in early-blocked asporogenous (Spo-) mutants of Bacillus subtilis 168, which were also defective in the production of antibiotics (Abs-), by temperate phage phi105 or SPO2 was studied. It was found that the frequency of lysogenization of Spo-Abs-mutants was 10 to 20% that of the wild-type bacteria. There was no difference in the efficiency of plating and the burst size of phi105 between wild-type and mutant strains. Phi105 lysogens of mutant strains were as stable as those of the wild type. Several rifampin-resistant mutants defective in the production of antibiotics were isolated. They were also defective in spore formation and lysogenized by phi105 at reduced frequency.

摘要

研究了枯草芽孢杆菌168早期受阻的无芽孢形成(Spo-)突变体(这些突变体在抗生素产生方面也存在缺陷,即Abs-)被温和噬菌体phi105或SPO2溶源化的情况。发现Spo-Abs-突变体的溶源化频率是野生型细菌的10%至20%。野生型和突变型菌株在phi105的平板接种效率和裂解量上没有差异。突变型菌株的phi105溶源菌与野生型的一样稳定。分离出了几个在抗生素产生方面有缺陷的利福平抗性突变体。它们在芽孢形成方面也有缺陷,并且被phi105溶源化的频率降低。

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REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.枯草芽孢杆菌转化的要求。
J Bacteriol. 1961 May;81(5):741-6. doi: 10.1128/jb.81.5.741-746.1961.

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