Ikeuchi T, Kurahashi K
J Bacteriol. 1978 May;134(2):440-5. doi: 10.1128/jb.134.2.440-445.1978.
The establishment of lysogeny in early-blocked asporogenous (Spo-) mutants of Bacillus subtilis 168, which were also defective in the production of antibiotics (Abs-), by temperate phage phi105 or SPO2 was studied. It was found that the frequency of lysogenization of Spo-Abs-mutants was 10 to 20% that of the wild-type bacteria. There was no difference in the efficiency of plating and the burst size of phi105 between wild-type and mutant strains. Phi105 lysogens of mutant strains were as stable as those of the wild type. Several rifampin-resistant mutants defective in the production of antibiotics were isolated. They were also defective in spore formation and lysogenized by phi105 at reduced frequency.
研究了枯草芽孢杆菌168早期受阻的无芽孢形成(Spo-)突变体(这些突变体在抗生素产生方面也存在缺陷,即Abs-)被温和噬菌体phi105或SPO2溶源化的情况。发现Spo-Abs-突变体的溶源化频率是野生型细菌的10%至20%。野生型和突变型菌株在phi105的平板接种效率和裂解量上没有差异。突变型菌株的phi105溶源菌与野生型的一样稳定。分离出了几个在抗生素产生方面有缺陷的利福平抗性突变体。它们在芽孢形成方面也有缺陷,并且被phi105溶源化的频率降低。