Bombick D W, Ayres P H, Putnam K, Bombick B R, Doolittle D J
Environmental and Molecular Toxicology Division, R. J. Reynolds Tobacco Company, Winston-Salem, NC 27102, USA.
Food Chem Toxicol. 1998 Mar;36(3):191-7. doi: 10.1016/s0278-6915(97)00155-5.
Mainstream smoke from Kentucky reference low "tar" (1R4F) and ultra-low "tar" (1R5F) cigarettes and a test cigarette (TOB-HT), that primarily heats tobacco, was compared for cytotoxic and genotoxic potential using cellular smoke exposure technology (CSET). CSET includes a computer controlled 30-port AMESA/Battelle-Geneva smoke generator which exposes cultured mammalian Chinese hamster ovary cells (CHO) to whole smoke. Cytotoxicity was assessed using the neutral red assay and genotoxicity was assessed using the sister chromatid exchange (SCE) assay. Compared on a per cigarette basis, mainstream smoke from 1R5F and the TOB-HT cigarette was significantly less cytotoxic and genotoxic than the smoke from the 1R4F cigarette. The cytotoxic and genotoxic activity of smoke from the TOB-HT cigarettes was slightly greater than the smoke from the ultra-low "tar" Kentucky 1R5F reference cigarettes. In conclusion, in these assays mainstream whole smoke of the TOB-HT cigarette had slightly greater cytotoxic and genotoxic potential compared with an ultra-low "tar" 1R5F Kentucky reference cigarette and significantly less activity compared with the whole mainstream smoke from a low "tar" 1R4F Kentucky reference cigarette, representative of the US market average cigarette for FTC yields of "tar", CO and nicotine.
使用细胞烟雾暴露技术（CSET），对肯塔基参考低“焦油”（1R4F）和超低“焦油”（1R5F）香烟以及一种主要加热烟草的测试香烟（TOB-HT）的主流烟雾的细胞毒性和遗传毒性潜力进行了比较。CSET包括一台计算机控制的30端口AMESA/巴特尔-日内瓦烟雾发生器，可将培养的哺乳动物中国仓鼠卵巢细胞（CHO）暴露于全烟雾中。使用中性红试验评估细胞毒性，使用姐妹染色单体交换（SCE）试验评估遗传毒性。按每支香烟计算，1R5F和TOB-HT香烟的主流烟雾的细胞毒性和遗传毒性明显低于1R4F香烟的烟雾。TOB-HT香烟烟雾的细胞毒性和遗传毒性活性略高于超低“焦油”肯塔基1R5F参考香烟的烟雾。总之，在这些试验中，与超低“焦油”肯塔基1R5F参考香烟相比，TOB-HT香烟的主流全烟雾具有略高的细胞毒性和遗传毒性潜力，与代表美国市场平均FTC“焦油”、一氧化碳和尼古丁产量的低“焦油”肯塔基1R4F参考香烟的主流全烟雾相比，活性明显较低。
