Vince A, Poljak M, Seme K
Dr Fran Mihaljević University Hospital for Infectious Diseases, Zagreb, Croatia.
Br J Haematol. 1998 May;101(2):349-51. doi: 10.1046/j.1365-2141.1998.00702.x.
The ability of six rapid DNA extraction procedures to provide DNA for the polymerase chain reaction from archival Giemsa-stained bone marrow slides was tested on 120 samples. Boiling in distilled water, freeze-thaw method, boiling in 10% Chelex-100 resin solution, proteinase K/Tween 20/NP-40 method coupled with simplified phenol/ chloroform/isoamyl alcohol protocol or salting-out procedure using saturated NaCl and modification of commercial QIAamp procedure (Qiagen. Chatsworth, Calif.) gave DNA extraction efficiencies of 50%, 70%, 85%, 95%, 100% and 100%, respectively. Our results demonstrate that rough DNA extraction methods have decreased efficiencies compared to complete DNA extraction protocols and that the latter are required to ensure highly reproducible results from archival Giemsa-stained bone marrow slides.
在120个样本上测试了六种快速DNA提取方法从存档的吉姆萨染色骨髓涂片为聚合酶链反应提供DNA的能力。在蒸馏水中煮沸、冻融法、在10% Chelex-100树脂溶液中煮沸、蛋白酶K/Tween 20/NP-40法结合简化的苯酚/氯仿/异戊醇方案或使用饱和NaCl的盐析程序以及对商业QIAamp程序（Qiagen，加利福尼亚州查茨沃思）的改进，DNA提取效率分别为50%、70%、85%、95%、100%和100%。我们的结果表明，与完整的DNA提取方案相比，粗略的DNA提取方法效率较低，并且需要后者来确保从存档的吉姆萨染色骨髓涂片中获得高度可重复的结果。
