Hallstrand T S, Ault K A, Bates P W, Mitchell J, Schoene R B
Maine Medical Center, Portland, USA.
Ann Allergy Asthma Immunol. 1998 May;80(5):424-32. doi: 10.1016/S1081-1206(10)62996-1.
Recently, TH2 lymphocyte activation has been shown to play a key role in initiating and propagating the inflammatory response in asthmatic airways. This is manifest through increased numbers of "activated" CD25-(IL-2R)-bearing T-helper cells and can be seen through the IL-5 driven recruitment of eosinophils and IL-4-mediated B-cell expression of CD23 (low affinity IgE receptor) and ultimately IgE production.
To gain a better understanding of the role of immune cells in asthma by describing the peripheral blood immune cell phenotypes in mild atopic asthma.
We enrolled 13 patients with mild atopic asthma and a group of seven nonatopic, nonasthmatic controls. Objective measures of lung function were obtained. The peripheral blood was analyzed by flow cytometry for specific cellular markers at rest and during the development of exercise induced bronchospasm.
At rest the number of CD23-bearing B cells (169/mL versus 117/mL; P = .05) and the number of CD25-bearing T cells (355/mL versus 237/mL; P = .03) were increased in the asthma group. There was a linear relationship between these two lymphocyte subsets and the maximum voluntary ventilation at rest (r = 0.56, P = .01 and r = 0.57, P = .01). With the development of exercise-induced bronchospasm there was a significantly greater increase in CD23-positive B cells (96.7/mL versus 59.7/mL; P = .05) and CD25-positive T cells (111.8/mL versus 45.1; P = .01) in the asthma group.
These data indicate that TH2 lymphocyte activation is manifested by increased numbers of CD23-bearing B cells and CD25-bearing T cells in the peripheral blood of patients with stable mild atopic asthma. Further, these immune cell subsets correlate with markers of resting lung function and increase in the peripheral blood early after the development of exercise-induced bronchospasm.
最近研究表明，TH2淋巴细胞激活在引发和传播哮喘气道炎症反应中起关键作用。这表现为携带“活化”CD25（白细胞介素-2受体）的T辅助细胞数量增加，并且可以通过白细胞介素-5驱动的嗜酸性粒细胞募集以及白细胞介素-4介导的B细胞表达CD23（低亲和力IgE受体）以及最终的IgE产生来观察到。
通过描述轻度特应性哮喘患者外周血免疫细胞表型，更好地了解免疫细胞在哮喘中的作用。
我们招募了13例轻度特应性哮喘患者和一组7名非特应性、非哮喘对照者。获取肺功能的客观指标。通过流式细胞术分析外周血中静息状态下以及运动诱发支气管痉挛发作期间的特定细胞标志物。
静息状态下，哮喘组中携带CD23的B细胞数量（169/毫升对117/毫升；P = 0.05）和携带CD25的T细胞数量（355/毫升对237/毫升；P = 0.03）增加。这两个淋巴细胞亚群与静息状态下的最大自主通气量之间存在线性关系（r = 0.56，P = 0.01和r = 0.57，P = 0.01）。随着运动诱发支气管痉挛的发生，哮喘组中CD23阳性B细胞（96.7/毫升对59.7/毫升；P = 0.05）和CD25阳性T细胞（111.8/毫升对45.1；P = 0.01）的增加明显更大。
这些数据表明，在稳定的轻度特应性哮喘患者外周血中，TH2淋巴细胞激活表现为携带CD23的B细胞和携带CD25的T细胞数量增加。此外，这些免疫细胞亚群与静息肺功能指标相关，并且在运动诱发支气管痉挛发作后早期外周血中数量增加。
