Chowdhury N, De Rycke P H
Z Parasitenkd. 1976 Aug 16;50(2):151-60. doi: 10.1007/BF00380519.
The distribution of neutral lipids and phospholipids in Hymenolepis microstoma has been studied using Fettrot, Sudan Black B, Sudan IV and copper phthalocyanin staining techniques. In the cysticercoid, neutral lipids are found in the outer membrane, the lining of the cysticercoid cavity, the tegument of the larval worm and the calcareous corpuscles. A decreasing gradient of phospholipids is found starting from the acellular layer, through the circular fibrous layer, the longitudinal fibrous layer, the adjacent dense zone and ending with the lining of the cysticercoid cavity. Phospholipids are also found in the calcareous corpuscles and the tegument of the larval worm. In the young adult (3 days p.i.) fat globules are first seen to accumulate in the last 2-3 proglottids. Until the 6th day p.i. they are found in the posterior third of the worm, surrounding developing gonads, but mostly concentrated along the transverse line. The mature proglottids contain fat, (a) in both granular and globular forms: in the folds of the uterus, sperm ducts, cirrus pouch and tegument (proximal cytoplasm), (b) in a diffuse form: in the vitellaria, ovary, testes and the tegument (distal cytoplasm). Pre-gravid and gravid proglottids show the largest fat globules. From the cleaving embryo to the fully developed oncosphere the concentrations of neutral lipids and phospholipids vary in form, intensity and location. In all strobilar forms of the parasite neutral lipids and phospholipids are found in the tegument and calcareous corpuscles. Although in H. microstoma lipid droplets are found in the excretory canals, all lipids in the proglottids are not absolutely waste products. From the results it would appear that they play a role in the maturation of gonads and transformation of the fertilized ovum to the oncosphere.
已使用油红O、苏丹黑B、苏丹IV和铜酞菁染色技术研究了微小膜壳绦虫中性脂质和磷脂的分布。在似囊尾蚴中，中性脂质存在于外膜、似囊尾蚴腔的内衬、幼虫虫体的皮层和石灰小体中。从无细胞层开始，经环形纤维层、纵行纤维层、相邻致密区，直至似囊尾蚴腔的内衬，磷脂含量呈递减梯度。磷脂也存在于石灰小体和幼虫虫体的皮层中。在感染后3天的年轻成虫中，首次观察到脂肪球积聚在最后2 - 3个节片中。直到感染后第6天，它们位于虫体后三分之一处，围绕发育中的生殖腺，但大多沿横线集中。成熟节片中含有脂肪，（a）呈颗粒状和球状：存在于子宫褶皱、输精管、阴茎囊和皮层（近端细胞质）中，（b）呈弥漫状：存在于卵黄腺、卵巢、睾丸和皮层（远端细胞质）中。未成熟和成熟节片显示出最大的脂肪球。从分裂的胚胎到完全发育的六钩蚴，中性脂质和磷脂的浓度在形式、强度和位置上有所不同。在寄生虫的所有节片形式中，中性脂质和磷脂都存在于皮层和石灰小体中。虽然在微小膜壳绦虫的排泄管中发现了脂滴，但节片中的所有脂质并非绝对都是废物。从结果来看，它们似乎在生殖腺成熟以及受精卵向六钩蚴的转化中发挥作用。
