Iwakura M, Tokushige M, Katsuki H, Muramatsu S
J Biochem. 1978 May;83(5):1387-94. doi: 10.1093/oxfordjournals.jbchem.a132048.
Screening of four malic enzymes--NAD-linked enzyme [EC 1.1.1.38], NAD, NADP-linked enzyme [EC 1.1.1.39], NADP-linked enzyme [EC 1.1.1.40], and D-malic enzyme--was carried out with cell-free extracts of the following 16 strains of bacteria by the aid of Sepharose 6B column chromatography: 9 strains of enteric bacteria, 3 strains of Pseudomonas, Alcaligenes faecalis, Agrobacterium tumefaciens, Rhodospirillum rubrum, and Clostridium tetanomorphum. All the strains tested contained at least one malic enzyme. The NADP-linked enzyme activity was found in all the strains except C. tetanomorphum, the NAD-linked enzyme activity in 12 strains--8 strains of enteric bacteria, 2 strains of Pseudomonas, Ag. tumefaciens, and C. tetanomorphum--and D-malic enzyme activity in 4 strains--A, aerogenes (IFO 3319 and 12059), Ps. fluorescens, and R. rubrum. The NADP-linked and NAD-linked enzyme activities of two strains of Pseudomonas were not separated by the chromatography. The available evidence suggested that the NAD, NADP-linked enzyme was not present in these 16 strains. The comparative studies of molecular, enzymatic, and serological properties of the malic enzymes in these 16 strains revealed a close similarity of the same types of malic enzymes among enteric bacteria.
利用琼脂糖6B柱色谱法，对以下16株细菌的无细胞提取物进行了四种苹果酸酶的筛选，这四种苹果酸酶分别是：NAD连接酶[EC 1.1.1.38]、NAD、NADP连接酶[EC 1.1.1.39]、NADP连接酶[EC 1.1.1.40]和D - 苹果酸酶。这16株细菌包括：9株肠道细菌、3株假单胞菌、粪产碱菌、根癌土壤杆菌、深红红螺菌和破伤风梭状芽孢杆菌。所有测试菌株至少含有一种苹果酸酶。除破伤风梭状芽孢杆菌外，所有菌株均检测到NADP连接酶活性；12株菌株检测到NAD连接酶活性，分别是8株肠道细菌、2株假单胞菌、根癌土壤杆菌和破伤风梭状芽孢杆菌；4株菌株检测到D - 苹果酸酶活性，分别是产气肠杆菌（IFO 3319和12059）、荧光假单胞菌和深红红螺菌。两种假单胞菌菌株的NADP连接酶和NAD连接酶活性在色谱分析中未分离。现有证据表明，这16株菌株中不存在NAD、NADP连接酶。对这16株菌株中苹果酸酶的分子、酶学和血清学特性进行的比较研究表明，肠道细菌中相同类型的苹果酸酶具有密切的相似性。
