Szymanski D B, Klis D A, Larkin J C, Marks M D
Department of Genetics and Cell Biology, and Plant Molecular and Genetics Institute, University of Minnesota, St. Paul, Minnesota 55108, USA.
Genetics. 1998 Jun;149(2):565-77. doi: 10.1093/genetics/149.2.565.
In Arabidopsis, the timing and spatial arrangement of trichome initiation is tightly regulated and requires the activity of the GLABROUS1 (GL1) gene. The COTYLEDON TRICHOME 1 (COT1) gene affects trichome initiation during late stages of leaf development and is described in this article. In the wild-type background, cot1 has no observable effect on trichome initiation. GL1 overexpression in wild-type plants leads to a modest number of ectopic trichomes and to a decrease in trichome number on the adaxial leaf surface. The cot1 mutation enhances GL1-overexpression-dependent ectopic trichome formation and also induces increased leaf trichome initiation. The expressivity of the cot1 phenotype is sensitive to cot1 and 35S::GL1 gene dosage, and the most severe phenotypes are observed when cot1 and 35S::GL1 are homozygous. The COT1 locus is located on chromosome 2 15.3 cM north of er. Analysis of the interaction between cot1, try, and 35S::GL1 suggests that COT1 is part of a complex signal transduction pathway that regulates GL1-dependent adoption of the trichome cell fate.
在拟南芥中，表皮毛起始的时间和空间排列受到严格调控，需要GLABROUS1（GL1）基因的活性。本文描述了子叶表皮毛1（COT1）基因，它在叶片发育后期影响表皮毛的起始。在野生型背景下，cot1对表皮毛起始没有可观察到的影响。野生型植物中GL1的过表达导致少量异位表皮毛，并使叶片近轴表面的表皮毛数量减少。cot1突变增强了GL1过表达依赖的异位表皮毛形成，还诱导叶片表皮毛起始增加。cot1表型的表达对cot1和35S::GL1基因剂量敏感，当cot1和35S::GL1均为纯合时观察到最严重的表型。COT1基因座位于第2号染色体上，在er以北15.3 cM处。对cot1、try和35S::GL1之间相互作用的分析表明，COT1是一个复杂信号转导途径的一部分，该途径调节GL1依赖的表皮毛细胞命运的形成。
