2'-5'寡核糖核苷酸的非酶促、模板导向连接。模板链与连接链的连接。
Non-enzymatic, template-directed ligation of 2'-5' oligoribonucleotides. Joining of a template and a ligator strand.
作者信息
Sawai H, Totsuka S, Yamamoto K, Ozaki H
机构信息
Department of Chemistry, Faculty of Engineering, Gunma University, Kiryu, Gunma 376-8515, Japan.
出版信息
Nucleic Acids Res. 1998 Jun 15;26(12):2995-3000. doi: 10.1093/nar/26.12.2995.
Abstract
Decauridylate containing exclusively a 2'-5' phospho-diester bond ([2'-5']U10) served as a template for the synthesis of oligoadenylates [oligo(A)s] from the 5'-phosphorimidazolide of 2'-5' diadenylate (ImpA-2'p5'A). Joining of [2'-5']U10and ImpA2'p5'A also took place in substantial amounts to yield long-chain oligoribonucleotides in the template-directed reaction. An unusual CD spectrum ascribed to helix formation between [2'-5']U10and 2'-5'2was observed under the same conditions as that of the template-directed reaction. The 3'-5' linked decauridylate ([3'-5']U10) also promoted the template-directed synthesis of oligo(A)s from ImpA2'p5'A, but more slowly compared with [2'-5']U10. The results indicate that short-chain RNA oligomers with a 2'-5' phosphodiester bond could lead to longer oligoribonucleotides by template-directed chain elongation.
摘要
仅含有2'-5'磷酸二酯键的十聚脱氧腺苷酸（[2'-5']U10）作为以2'-5'二腺苷酸的5'-磷酰咪唑酯（ImpA-2'p5'A）合成寡聚腺苷酸[oligo(A)s]的模板。在模板导向反应中，[2'-5']U10与ImpA2'p5'A也大量连接，生成了长链寡聚核糖核苷酸。在与模板导向反应相同的条件下，观察到了一种归因于[2'-5']U10与2'-5'2之间形成螺旋的异常圆二色光谱。3'-5'连接的十聚脱氧腺苷酸（[3'-5']U10）也促进了从ImpA2'p5'A进行模板导向的oligo(A)s合成，但与[2'-5']U10相比更慢。结果表明，具有2'-5'磷酸二酯键的短链RNA寡聚物可通过模板导向的链延伸导致生成更长的寡聚核糖核苷酸。
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