• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

毛细管电泳在配体 - DNA相互作用研究中的应用。

Use of capillary electrophoresis in the study of ligand-DNA interactions.

作者信息

Hamdan I I, Skellern G G, Waigh R D

机构信息

Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow G1 1XW, UK.

出版信息

Nucleic Acids Res. 1998 Jun 15;26(12):3053-8. doi: 10.1093/nar/26.12.3053.

DOI:10.1093/nar/26.12.3053
PMID:9611254
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147652/
Abstract

Free solution capillary electrophoresis (FSCE) has been used to separate two non-self-complementary 12mer oligonucleotide duplexes: d(AAATTATATTAT).d(ATAA-TATAATTT) and d(GGGCCGCGCCGC).d(GCGGCGCGGCCC). Titration of mixtures of the two oligonucleotides with model intercalators (ethidium bromide andactinomycin D) and minor groove binders (netropsin, Hoechst 33258 and distamycin) has shown the suitability of FSCE as a method to study the sequence selectivity of DNA binding agents. Binding data have shown cooperativity of binding for netropsin and Hoechst 33258 and have provided ligand:DNA binding ratios for all five compounds. Cooperativity of netropsin binding to a 12mer with two potential sites has been demonstrated for the first time. Ligands binding in the minor groove caused changes in migration time and peak shape which were significantly different from those caused by intercalators.

摘要

自由溶液毛细管电泳（FSCE）已被用于分离两种非自互补的12聚体寡核苷酸双链体：d(AAATTATATTAT).d(ATAA-TATAATTT) 和 d(GGGCCGCGCCGC).d(GCGGCGCGGCCC)。用模型嵌入剂（溴化乙锭和放线菌素D）和小沟结合剂（纺锤菌素、Hoechst 33258和偏端霉素）对这两种寡核苷酸的混合物进行滴定，结果表明FSCE适合作为研究DNA结合剂序列选择性的方法。结合数据显示了纺锤菌素和Hoechst 33258结合的协同性，并提供了所有五种化合物的配体与DNA的结合比率。首次证明了纺锤菌素与具有两个潜在位点的12聚体结合的协同性。在小沟中结合的配体导致迁移时间和峰形的变化，这些变化与嵌入剂引起的变化显著不同。

相似文献

1
Use of capillary electrophoresis in the study of ligand-DNA interactions.毛细管电泳在配体 - DNA相互作用研究中的应用。
Nucleic Acids Res. 1998 Jun 15;26(12):3053-8. doi: 10.1093/nar/26.12.3053.
2
Ligand binding to oligonucleotides.配体与寡核苷酸的结合。
Methods Mol Biol. 2001;163:379-91. doi: 10.1385/1-59259-116-7:379.
3
Binding of distamycin A and netropsin to the 12mer DNA duplexes containing mixed AT.GC sequences with at most five or three successive AT base pairs.放线菌素A和纺锤菌素与含有混合AT.GC序列且至多有五个或三个连续AT碱基对的12聚体DNA双链体的结合。
Biochemistry. 2000 Aug 8;39(31):9317-26. doi: 10.1021/bi000748u.
4
DNA sequence preferences of several AT-selective minor groove binding ligands.几种AT选择性小沟结合配体的DNA序列偏好性。
Nucleic Acids Res. 1995 Sep 11;23(17):3385-92. doi: 10.1093/nar/23.17.3385.
5
Molecular dynamics simulations and free energy calculations of netropsin and distamycin binding to an AAAAA DNA binding site.纺锤菌素和偏端霉素与AAAAA DNA结合位点结合的分子动力学模拟及自由能计算
Nucleic Acids Res. 2005 Feb 1;33(2):725-33. doi: 10.1093/nar/gki195. Print 2005.
6
Molecular modelling of the interaction of carbocyclic analogues of netropsin and distamycin with d(CGCGAATTCGCG)2.纺锤菌素和偏端霉素的碳环类似物与d(CGCGAATTCGCG)2相互作用的分子模拟
Acta Biochim Pol. 2000;47(3):855-66.
7
Interaction between antitumor drugs and a double-stranded oligonucleotide studied by electrospray ionization mass spectrometry.通过电喷雾电离质谱法研究抗肿瘤药物与双链寡核苷酸之间的相互作用。
J Mass Spectrom. 1999 Dec;34(12):1328-37. doi: 10.1002/(SICI)1096-9888(199912)34:12<1328::AID-JMS889>3.0.CO;2-F.
8
Heterogeneity in the actions of drugs that bind in the DNA minor groove.结合于DNA小沟的药物作用的异质性。
Biochemistry. 1999 Aug 3;38(31):10135-46. doi: 10.1021/bi990382p.
9
Energetic diversity of DNA minor-groove recognition by small molecules displayed through some model ligand-DNA systems.通过一些模型配体 - DNA 系统展示的小分子对 DNA 小沟识别的能量多样性。
J Mol Biol. 2004 Sep 3;342(1):73-89. doi: 10.1016/j.jmb.2004.07.005.
10
Minor groove binding ligands alter the rotational positioning of DNA fragments on nucleosome core particles.小沟结合配体改变了DNA片段在核小体核心颗粒上的旋转定位。
J Mol Biol. 1996 Oct 11;262(5):671-85. doi: 10.1006/jmbi.1996.0544.

引用本文的文献

1
Nucleic acid binding affinity and antioxidant activity of N-m-Tolyl-4-Chlorophenoxyacetohydroxamicacid.N-甲基-4-氯苯氧乙酰基羟肟酸的核酸结合亲和力和抗氧化活性。
Sci Rep. 2024 Sep 28;14(1):22465. doi: 10.1038/s41598-024-72477-x.
2
Ranking ligand affinity for the DNA minor groove by experiment and simulation.通过实验和模拟对DNA小沟的配体亲和力进行排序。
ACS Med Chem Lett. 2010 Jul 30;1(8):376-80. doi: 10.1021/ml100047n. eCollection 2010 Nov 11.
3
Effect of the matrix on DNA electrophoretic mobility.基质对DNA电泳迁移率的影响。
J Chromatogr A. 2009 Mar 6;1216(10):1917-29. doi: 10.1016/j.chroma.2008.11.090. Epub 2008 Dec 6.
4
Internal 32P-labeling of L-deoxyoligonucleotides.L-脱氧寡核苷酸的内部32P标记
Nucleic Acids Res. 2003 Apr 1;31(7):e34. doi: 10.1093/nar/gng034.
5
Massive parallel analysis of the binding specificity of histone-like protein HU to single- and double-stranded DNA with generic oligodeoxyribonucleotide microchips.利用通用寡脱氧核糖核苷酸微芯片对组蛋白样蛋白HU与单链和双链DNA结合特异性进行大规模平行分析。
Nucleic Acids Res. 2001 Jun 15;29(12):2654-60. doi: 10.1093/nar/29.12.2654.
6
Sequence-dependent variation in DNA minor groove width dictates orientational preference of Hoechst 33258 in A-tract recognition: solution NMR structure of the 2:1 complex with d(CTTTTGCAAAAG)(2).DNA小沟宽度的序列依赖性变化决定了Hoechst 33258在A-序列识别中的取向偏好：与d(CTTTTGCAAAAG)(2)形成的2:1复合物的溶液核磁共振结构
Nucleic Acids Res. 2000 Feb 1;28(3):728-35. doi: 10.1093/nar/28.3.728.

本文引用的文献

1
Conformation dependent binding of netropsin and distamycin to DNA and DNA model polymers.纺锤菌素和偏端霉素与DNA及DNA模型聚合物的构象依赖性结合
Nucleic Acids Res. 1974 Mar;1(3):503-30. doi: 10.1093/nar/1.3.503.
2
Separation of pd(GC)12 from pd(AT)12 by free solution capillary electrophoresis.通过自由溶液毛细管电泳从pd(AT)12中分离出pd(GC)12。
J Chromatogr A. 1998 May 8;806(1):165-8. doi: 10.1016/s0021-9673(97)00504-9.
3
Interaction of minor groove ligands to an AAATT/AATTT site: correlation of thermodynamic characterization and solution structure.小沟配体与AAATT/AATTT位点的相互作用：热力学表征与溶液结构的相关性
Biochemistry. 1995 Mar 7;34(9):2937-45. doi: 10.1021/bi00009a025.
4
DNA sequence preferences of several AT-selective minor groove binding ligands.几种AT选择性小沟结合配体的DNA序列偏好性。
Nucleic Acids Res. 1995 Sep 11;23(17):3385-92. doi: 10.1093/nar/23.17.3385.
5
Determination of antigen-antibody affinity by immunocapillary electrophoresis.
J Chromatogr A. 1994 Oct 7;680(2):405-12. doi: 10.1016/0021-9673(94)85136-0.
6
Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.通过抗DNase I分析放线菌素D和纺锤菌素与pBR322 DNA结合的序列特异性
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3260-4. doi: 10.1073/pnas.80.11.3260.
7
Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II).异质DNA上偏端霉素、纺锤菌素和放线菌素结合位点的图谱：甲基丙基乙二胺四乙酸铁（II）的DNA切割抑制模式
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5470-4. doi: 10.1073/pnas.79.18.5470.
8
Spectroscopic analysis of drug-nucleic acid interactions.
CRC Crit Rev Biochem. 1982 Feb;12(2):103-32. doi: 10.3109/10409238209108704.
9
Interaction of the oligopeptide antibiotics netropsin and distamycin A with nucleic acids.寡肽抗生素纺锤菌素和偏端霉素A与核酸的相互作用。
J Mol Biol. 1971 May 28;58(1):329-48. doi: 10.1016/0022-2836(71)90250-6.
10
Footprinting at low temperatures: evidence that ethidium and other simple intercalators can discriminate between different nucleotide sequences.低温下的足迹分析：溴化乙锭及其他简单嵌入剂可区分不同核苷酸序列的证据。
Nucleic Acids Res. 1987 Jan 26;15(2):491-507. doi: 10.1093/nar/15.2.491.