Fox K R, Waring M J
Nucleic Acids Res. 1987 Jan 26;15(2):491-507. doi: 10.1093/nar/15.2.491.
Footprinting experiments employing DNAase I have been performed at 4 degrees C. At this temperature several simple intercalating ligands, including both ethidium and proflavine, can be seen to induce marked changes in the pattern of cleavage. From an analysis of the changes in patterns of digestion by DNAase I we deduce that ethidium binds best to regions of mixed nucleotide sequence, especially those containing alternating purines and pyrimidines. Binding seems to be weakest to poly dA sequences which consequently appear as regions of relatively enhanced cleavage. Attempts to reproduce these changes using DNAase II as a footprinting tool were unsuccessful.
利用脱氧核糖核酸酶I进行的足迹实验是在4摄氏度下进行的。在此温度下,可以看到几种简单的嵌入配体,包括溴化乙锭和原黄素,会诱导切割模式发生显著变化。通过分析脱氧核糖核酸酶I消化模式的变化,我们推断溴化乙锭与混合核苷酸序列区域结合最佳,尤其是那些含有交替嘌呤和嘧啶的区域。与聚dA序列的结合似乎最弱,因此这些区域表现为切割相对增强的区域。使用脱氧核糖核酸酶II作为足迹工具来重现这些变化的尝试未成功。