Taji S S, Rogers A H
Department of Dentistry, University of Adelaide.
Aust Dent J. 1998 Apr;43(2):128-30. doi: 10.1111/j.1834-7819.1998.tb06101.x.
Ten individuals were each supplied with a new toothbrush of the same type and brand, together with identical tubes of fluoridated toothpaste. After a three-week period, during which subjects were asked to follow their usual oral hygiene practices, the toothbrushes were collected and assayed for microbial contamination using a range of selective growth media. The total microbial load per toothbrush was found to be 10(4) to 10(5) colony forming units. Staphylococci were found on all toothbrushes and streptococci on all but one. These two genera were also quantitatively dominant. Candida, corynebacteria, pseudomonads and coliforms were identified in 70, 60, 50 and 30 per cent of toothbrushes, respectively. However, mutans streptococci, lactobacilli and black-pigmented Gram-negative anaerobic rods were not detected on any of the toothbrushes. For each individual, information on variables such as toothbrush rinsing practices and post-brushing storage methods and environment was collected. No obvious relationship between such variables and microbial load was apparent but it is suggested that more extensive studies are needed, taking into account additional parameters such as age and degree of toothbrush wear and the use of pre-brushing mouthwashes.
给10个人每人提供了一支相同类型和品牌的新牙刷，以及相同的含氟牙膏管。在为期三周的时间里，要求受试者遵循他们通常的口腔卫生习惯，之后收集牙刷，并使用一系列选择性生长培养基对其进行微生物污染检测。发现每支牙刷的总微生物负荷为10⁴至10⁵菌落形成单位。所有牙刷上都发现了葡萄球菌，除了一支牙刷外，其他所有牙刷上都发现了链球菌。这两个属在数量上也占主导地位。分别在70%、60%、50%和30%的牙刷中鉴定出念珠菌、棒状杆菌、假单胞菌和大肠菌群。然而，在任何一支牙刷上都未检测到变形链球菌、乳酸杆菌和黑色色素革兰氏阴性厌氧棒状菌。对于每个人，收集了诸如牙刷冲洗习惯、刷牙后储存方法和环境等变量的信息。这些变量与微生物负荷之间没有明显的关系，但建议进行更广泛的研究，同时考虑年龄、牙刷磨损程度和刷牙前使用漱口水等其他参数。
