与猪CD8反应的单克隆抗体分析。
Analyses of mAb reactive with porcine CD8.
作者信息
Saalmüller A, Aasted B, Canals A, Dominguez J, Goldman T, Lunney J K, Maurer S, Pescovitz M D, Pospisil R, Salmon H
机构信息
Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
出版信息
Vet Immunol Immunopathol. 1994 Oct;43(1-3):249-54. doi: 10.1016/0165-2427(94)90144-9.
Among all mAb submitted to the first porcine CD workshop, based on FCM analyses six mAb could be identified to recognize the porcine CD8 analogue (workshop Nos. 004, 051, 052, 053, 108 and 109). In immunoprecipitation studies three mAb (Nos. 004, 108 and 109) recognized an antigen with an apparent molecular mass of about 35 kDa under reducing conditions and about 70 kDa under non-reducing conditions. The molecular masses of the antigens recognized by the three other mAb (Nos. 051, 052 and 053) are still unknown. Epitope analyses performed by blocking experiments led to the determination of two CD8 epitopes: CD8a and CD8b. CD8a is recognized by mAb Nos. 004, 051 and 052, and CD8b by Nos. 053, 108 and 109.
在提交给首届猪CD研讨会的所有单克隆抗体中，基于流式细胞术分析，可鉴定出六种单克隆抗体识别猪CD8类似物（研讨会编号004、051、052、053、108和109）。在免疫沉淀研究中，三种单克隆抗体（编号004、108和109）在还原条件下识别一种表观分子量约为35 kDa的抗原，在非还原条件下识别约70 kDa的抗原。其他三种单克隆抗体（编号051、052和053）识别的抗原分子量仍未知。通过阻断实验进行的表位分析确定了两个CD8表位：CD8a和CD8b。CD8a被编号004、051和052的单克隆抗体识别，CD8b被编号053、108和109的单克隆抗体识别。
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