Zimmermann B, Adhami H
Acta Anat (Basel). 1976;95(3):399-407. doi: 10.1159/000144628.
Studies were carried out on the nuclear size of embryonic mouse brain monolayer cells at various cell densities. The variation in cell density resulted from different initial cell numbers. The nuclear area was measured area-analytically with the Quantimet, the determination of cell population was measured linear-analytically using the Digiscan. Nuclear size remains constant in a cell culture of 10(4) up to 8.3 X 10(4) cells ml, which corresponds to a cell density of about 25-160 cells/unit. A significant diminution in mean nuclear size of the cell nucleus occurs in the case of increased cell densities of 215 and 277 cells/unit, corresponding to a cell culture of 2.5 X 10(5) AND 7.3 X 10(5) cells ml. This diminution may be connected with the accumulation of G1 cell nuclei in cultures with contact-dependent growth inhibition.
对不同细胞密度下胚胎小鼠脑单层细胞的细胞核大小进行了研究。细胞密度的变化源于不同的初始细胞数量。使用图像分析系统（Quantimet）通过面积分析测量核面积，使用数字扫描系统（Digiscan）通过线性分析测量细胞群体数量。在细胞密度为10⁴至8.3×10⁴个细胞/毫升的细胞培养物中，细胞核大小保持恒定，这相当于细胞密度约为25 - 160个细胞/单位。当细胞密度增加到215和277个细胞/单位时，细胞核的平均大小显著减小，这相当于细胞培养物中细胞密度为2.5×10⁵和7.3×10⁵个细胞/毫升。这种减小可能与接触依赖性生长抑制培养物中G1期细胞核的积累有关。
