Adhami H, Zimmermann B
Arzneimittelforschung. 1976;26(1):22-6.
Actinomycin D (AMD) inhibits the RNA-synthesis by intercalating with the DNA-helix. In monolayer culture of embryonic mouse brain cells in the 8th passage the cell nuclei were demonstrated with the Feulgen-reaction and quantitatively-morphometrically evaluated following a 48 h intoxication with AMD (0.001; 0.01; 0.1 mug/ml). The mean value of nuclear area calculated from single measurements with the Quantimet 720 is significantly larger when compared to the controls at a dose of 0.01 mug AMD/ml. At 0.1 mug AMD/ml ca. 30% of the cell nuclei are pyknotic; the non-pyknotic nuclei are enlarged significantly by appr. 20%. The linear-analytic results using a Digiscan show no enlargement of tendon length, on the contrary, at 0.1 mug AMD/ml a significant diminution versus the controls can be seen. This resutls from the pyknotic cell nuclei which are also measured in this case no single measurements were carried out. The determination of cell density using a Digiscan shows a diminution of cell number by 19, 31 or 68% dependent upon AMD-concentration.
放线菌素D（AMD）通过嵌入DNA螺旋来抑制RNA合成。在第8代小鼠胚胎脑细胞的单层培养中，用福尔根反应显示细胞核，并在AMD（0.001；0.01；0.1微克/毫升）中毒48小时后进行定量形态学评估。用Quantimet 720单次测量计算出的核面积平均值在AMD剂量为0.01微克/毫升时与对照组相比显著更大。在AMD为0.1微克/毫升时，约30%的细胞核固缩；未固缩的细胞核明显增大约20%。使用Digiscan的线性分析结果显示肌腱长度没有增加，相反，在AMD为0.1微克/毫升时，与对照组相比有显著减小。这是由于固缩的细胞核也在这种情况下被测量，没有进行单次测量。使用Digiscan测定细胞密度显示，细胞数量根据AMD浓度减少了19%、31%或68%。
