Rahman I, Smith C A, Antonicelli F, MacNee W
Department of Medicine, University of Edinburgh, Royal Infirmary, UK.
FEBS Lett. 1998 May 1;427(1):129-33. doi: 10.1016/s0014-5793(98)00410-4.
The 5'-flanking region of human gamma-glutamylcysteine synthetase-heavy subunit (gamma-GCS-HS) was characterised by creating a series of chloramphenicol acetyl transferase (CAT) reporter deletion constructs. Analysis of various deleted CAT constructs revealed that a putative AP-1 consensus sequence is required to direct the constitutive and oxidant-mediated promoter activity. Gel mobility shift and mutation analysis of the sequence (-269 to -263 bp), showed binding of AP-1 is involved in the oxidant-mediated regulation of gamma-GCS-HS promoter activity.
通过构建一系列氯霉素乙酰转移酶（CAT）报告基因缺失构建体，对人γ-谷氨酰半胱氨酸合成酶重亚基（γ-GCS-HS）的5'-侧翼区域进行了表征。对各种缺失的CAT构建体的分析表明，一个假定的AP-1共有序列是指导组成型和氧化剂介导的启动子活性所必需的。对序列（-269至-263 bp）进行凝胶迁移率变动分析和突变分析，结果显示AP-1的结合参与了氧化剂介导的γ-GCS-HS启动子活性调控。
