McNabb Scott J N, Braden Christopher R, Navin Thomas R
Centers for Disesase Control and Prevention, Atlanta, GA 30333, USA.
Emerg Infect Dis. 2002 Nov;8(11):1314-9. doi: 10.3201/eid0811.020402.
DNA fingerprinting of Mycobacterium tuberculosis--a relatively new laboratory technique--offers promise as a powerful aid in the prevention and control of tuberculosis (TB). Established in 1996 by the Centers for Disease Control and Prevention (CDC), the National Tuberculosis Genotyping and Surveillance Network was a 5-year prospective, population-based study of DNA fingerprinting conducted from 1996 to 2000. The data from this study suggest multiple molecular epidemiologic and program management uses for DNA fingerprinting in TB public health practice. From these data, we also gain a clearer understanding of the overall diversity of M. tuberculosis strains as well as the presence of endemic strains in the United States. We summarize the key findings and the impact that DNA fingerprinting may have on future approaches to TB control. Although challenges and limitations to the use of DNA fingerprinting exist, the widespread implementation of the technique into routine TB prevention and control practices appears scientifically justified.
结核分枝杆菌的DNA指纹识别技术——一项相对较新的实验室技术——有望成为预防和控制结核病的有力辅助手段。国家结核病基因分型与监测网络由疾病控制与预防中心（CDC）于1996年设立，是一项从1996年至2000年开展的为期5年的前瞻性、基于人群的DNA指纹识别研究。该研究数据表明，DNA指纹识别技术在结核病公共卫生实践中有多种分子流行病学及项目管理用途。通过这些数据，我们还能更清楚地了解结核分枝杆菌菌株的总体多样性以及美国本土菌株的情况。我们总结了关键发现以及DNA指纹识别技术可能对未来结核病控制方法产生的影响。尽管使用DNA指纹识别技术存在挑战和局限性，但将该技术广泛应用于常规结核病预防和控制实践在科学上似乎是合理的。
