Levin B C, Freese E
Laboratory of Molecular Biology, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, Maryland.
J Environ Sci Health C. 1978;13(1):1-31.
More than 80% of the hexachlorophene added to a Bacillus subtilis culture binds to the cells. Complete growth inhibition requires 6 x 10(5) molecules bound per cell. In contrast, more than 99% decanoate remains in solution and 3.8 x 10(7) molecules bound per cell are needed to inhibit growth. Centrifugation and resuspension of cells in growth medium removes only decanoate, whereas the addition of 1% bovine serum albumin to the growth medium removes both inhibitors from their binding sites on the cells. The addition of untreated cells to a hexachlorophene-treated culture enables the hexachlorophene molecules to redistribute among all the cells with the result that the inhibited cells can resume growth.
添加到枯草芽孢杆菌培养物中的六氯酚，超过80%会与细胞结合。完全抑制生长需要每个细胞结合6×10⁵个分子。相比之下，超过99%的癸酸盐仍留在溶液中，抑制生长需要每个细胞结合3.8×10⁷个分子。细胞在生长培养基中进行离心和重悬只能去除癸酸盐，而在生长培养基中添加1%的牛血清白蛋白则能将两种抑制剂从细胞上的结合位点去除。将未处理的细胞添加到经六氯酚处理的培养物中，可使六氯酚分子在所有细胞中重新分布，结果是受抑制的细胞能够恢复生长。
