Larsson C T, Khoshnoodi J, Ek B, Rask L, Larsson H
Uppsala Genetic Center, Dept. of Cell Research, Swedish University of Agricultural Sciences.
Plant Mol Biol. 1998 Jun;37(3):505-11. doi: 10.1023/a:1005908305456.
Full-length cDNA for starch branching enzyme (SBE) II of potato was isolated and sequenced. In potato, similarly to most other investigated plants, the SBE-II isoform differs from SBE-I by having an acidic amino-terminal extension and a shorter carboxyterminus. Two forms of SBE-II, migrating as 98 and 95 kDa proteins in 6% SDS-polyacrylamide gels, were associated to tuber starch. The latter form was 16 amino acids shorter in the amino terminus. Transcript of SBE-II was present in leaf tissue, whereas significant expression was not seen in tubers. On the other hand, a significant amount of SBE-I transcript was detected in tuber tissue but not in leaves.
分离并测序了马铃薯淀粉分支酶（SBE）II的全长cDNA。在马铃薯中，与大多数其他已研究的植物类似，SBE-II同工型与SBE-I的不同之处在于其具有酸性氨基末端延伸和较短的羧基末端。在6%十二烷基硫酸钠-聚丙烯酰胺凝胶中迁移为98 kDa和95 kDa蛋白质的两种SBE-II形式与块茎淀粉相关。后一种形式的氨基末端短16个氨基酸。SBE-II的转录本存在于叶片组织中，而在块茎中未观察到显著表达。另一方面，在块茎组织中检测到大量SBE-I转录本，但在叶片中未检测到。
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