正常玉米和蜡质玉米中颗粒结合型淀粉合酶的部分纯化和性质鉴定。
Partial purification and characterization of granule-bound starch synthases from normal and waxy maize.
机构信息
Biochemistry/Biophysics Department, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1985 Aug;78(4):849-52. doi: 10.1104/pp.78.4.849.
Abstract
The granule-bound starch-synthases from normal and waxy maize kernels have been solubilized, partially purified, and characterized. Two broad categories of starch synthases were revealed with representatives in the soluble phase and also on granules from both normal and waxy maize though the activity associated with granules from waxy was small. Data for native molecular weights, kinetic parameters, and immunological relatedness are used to demonstrate that the granule-bound isozymes from normal maize are different from the soluble enzymes. These distinct granule-bound enzymes are missing in waxy maize granules and a further novel form of starch synthase is revealed. These findings are discussed in relation to the type of starch produced in tissue affected by the waxy mutation.
摘要
已经对正常玉米和蜡质玉米颗粒中的颗粒结合型淀粉合酶进行了溶解、部分纯化和特性分析。结果揭示了两类广泛的淀粉合酶，它们的代表分别存在于正常玉米和蜡质玉米的可溶性相和颗粒相中，尽管与蜡质玉米颗粒相关的活性很小。利用天然分子量、动力学参数和免疫学相关性的数据，证明了正常玉米颗粒结合型同工酶与可溶性酶不同。这些不同的颗粒结合型酶在蜡质玉米颗粒中缺失，并揭示了一种新的淀粉合酶形式。这些发现与受蜡质突变影响的组织中产生的淀粉类型有关。
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