Finkelman F D, Lipsky P E
J Immunol. 1978 May;120(5):1465-72.
The effects of F(ab')2 fragments of affinity-purified rabbit anti-human mu chain antibody (RaHmu) and rabbit anti-human delta chain antibody (RaHdelta) on spontaneous and mitogen-stimulated immunoglobulin (Ig) secretion by normal human spleen cells were studied. IgM and IgG secretion by human spleen cells cultured in vitro was measured by incubating the cells with 3H-amino acids precipitating the secreted labeled Ig with anti-Ig, and analyzing the precipitates by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Both RaHmu and RaHdelta suppressed spontaneous and LPS-induced IgM and IgG secretion as well as PWM-stimulated IgG secretion. In different experiments, RaHmu and RaHdelta either suppressed or augmented PWM-induced IgM secretion. The anti-Ig induced augmentation of PWM-triggered IgM secretion was most apparent when spleen cells were cultured at lower cell densities or when lower concentrations of anti-Ig were employed. These date indicate that perturbation of B cell surface immunoglobulin receptors with specific anti-Ig antibody can alter markedly the ability of these cells to differentiate into antibody-secreting cells.
研究了亲和纯化的兔抗人μ链抗体（RaHmu）和兔抗人δ链抗体（RaHdelta）的F(ab')2片段对正常人脾细胞自发分泌和丝裂原刺激的免疫球蛋白（Ig）分泌的影响。通过用3H-氨基酸孵育细胞，并用抗Ig沉淀分泌的标记Ig，然后通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析沉淀物，来测量体外培养的人脾细胞分泌的IgM和IgG。RaHmu和RaHdelta均抑制自发分泌和LPS诱导的IgM和IgG分泌以及PWM刺激的IgG分泌。在不同实验中，RaHmu和RaHdelta要么抑制要么增强PWM诱导的IgM分泌。当脾细胞以较低细胞密度培养或使用较低浓度的抗Ig时，抗Ig诱导的PWM触发的IgM分泌增强最为明显。这些数据表明，用特异性抗Ig抗体干扰B细胞表面免疫球蛋白受体可显著改变这些细胞分化为抗体分泌细胞的能力。
