Harrus S, Waner T, Keysary A, Aroch I, Voet H, Bark H
Department of Clinical Sciences, School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel.
Vet Immunol Immunopathol. 1998 Mar 18;62(1):15-27. doi: 10.1016/s0165-2427(97)00127-x.
In order to determine the role of the spleen in the pathogenesis of canine monocytic ehrlichiosis (CME), the effect of splenectomy on the course of the acute phase of experimental was investigated. Intact and splenectomized dogs, sero-negative for Ehrlichia canis antibodies, were infected with the Israeli strain of E. canis. Serology, clinical signs and haematological parameters were recorded prior to infection, and over a period of 60 days post infection, and were compared between the intact and the splenectomized dogs. All dogs seroconverted for IFA E. canis antibodies by days 10 to 17 post infection. There did not appear to be any difference in the day of appearance or in the titer of anti-E. canis IgG antibodies, between the splenectomized and intact groups throughout the course of the study. During the acute stage, food consumption (percentage change) was significantly lower in the intact group compared to the splenectomized group (-66.3% and -25.3%, respectively, p < 0.0001). During this period, significant higher body temperatures were measured in the intact group (average of 39.76 degrees C vs. 38.96 degrees C, p < 0.0001). The haematocrit, red blood cell counts, haemoglobin concentrations and platelet counts were significantly lower (p < 0.05) in the intact group when compared to the splenectomized group during the whole course of the study. The clinical and the haematological findings in our study suggest that the disease process was milder in the splenectomized dogs compared to the intact dogs. The results of this study suggest that the spleen plays an important role in the pathogenesis of CME. Splenic inflammatory mediators and/or other splenic substances, are proposed to play a key role in the pathogenesis of the disease. Our results further substantiate the involvement of immune mechanisms in the pathogenesis of CME.
为了确定脾脏在犬单核细胞埃立克体病（CME）发病机制中的作用，研究了脾切除对实验急性期病程的影响。对犬埃立克体抗体血清学阴性的完整和脾切除犬，用以色列株犬埃立克体进行感染。在感染前、感染后60天内记录血清学、临床症状和血液学参数，并在完整犬和脾切除犬之间进行比较。所有犬在感染后第10至17天IFA犬埃立克体抗体血清阳转。在整个研究过程中，脾切除组和完整组之间抗犬埃立克体IgG抗体出现的天数或滴度似乎没有任何差异。在急性期，完整组的食物消耗量（百分比变化）显著低于脾切除组（分别为-66.3%和-25.3%，p<0.0001）。在此期间，完整组测量的体温显著更高（平均39.76℃对38.96℃，p<0.0001）。在整个研究过程中，完整组的血细胞比容、红细胞计数、血红蛋白浓度和血小板计数与脾切除组相比显著更低（p<0.05）。我们研究中的临床和血液学结果表明，与完整犬相比，脾切除犬的疾病过程更轻。本研究结果表明，脾脏在CME的发病机制中起重要作用。脾炎性介质和/或其他脾脏物质被认为在该疾病的发病机制中起关键作用。我们的结果进一步证实了免疫机制参与了CME的发病过程。
