Bienkowski P, Piasecki J, Koros E, Stefanski R, Kostowski W
Department of Pharmacology and Physiology of the Nervous System, Institute of Psychiatry and Neurology, Warsaw, Poland.
Eur Neuropsychopharmacol. 1998 May;8(2):79-87. doi: 10.1016/s0924-977x(97)00052-7.
The role of the nicotinic acetylcholine receptor (nAChR) in the discriminative and aversive stimulus effects of ethanol was studied in rats. In the operant drug discrimination procedure the rats were trained to discriminate between 1.0 g/kg ethanol and saline under the FR10 schedule of sweetened milk reinforcement. Neither the nAChR agonist, nicotine (0.1-0.6 mg/kg) nor the nAChR antagonist, mecamylamine (3.0-6.0 mg/kg) substituted for the ethanol stimulus. Moreover, mecamylamine (0.5-6.0 mg/kg) did not antagonise the ethanol stimulus. The cross-familiarisation conditioned taste aversion procedure was used as an alternative method to study stimulus resemblance between ethanol and nicotine. Six daily injections of nicotine (0.6 mg/kg) significantly decreased a subsequent ethanol-induced taste aversion conditioning. The aversive stimulus effects of ethanol were investigated with the conditioned taste aversion (CTA) paradigm. Mecamylamine (1.0-3.0 mg/kg) did not attenuate an ethanol-induced CTA. These results suggest that: (1) nAChRs are not primarily involved in the discriminative stimulus effects of ethanol when studied with the operant drug discrimination test; (2) nAChRs are not critically involved in the ethanol-induced CTA.
在大鼠中研究了烟碱型乙酰胆碱受体（nAChR）在乙醇辨别性和厌恶性刺激效应中的作用。在操作性药物辨别程序中，训练大鼠在FR10强化甜牛奶的条件下区分1.0 g/kg乙醇和生理盐水。烟碱型乙酰胆碱受体激动剂尼古丁（0.1 - 0.6 mg/kg）和烟碱型乙酰胆碱受体拮抗剂美加明（3.0 - 6.0 mg/kg）均不能替代乙醇刺激。此外，美加明（0.5 - 6.0 mg/kg）不能拮抗乙醇刺激。交叉熟悉化条件性味觉厌恶程序被用作研究乙醇和尼古丁之间刺激相似性的另一种方法。连续6天注射尼古丁（0.6 mg/kg）显著降低了随后乙醇诱导的味觉厌恶条件反射。用条件性味觉厌恶（CTA）范式研究了乙醇的厌恶性刺激效应。美加明（1.0 - 3.0 mg/kg）不能减弱乙醇诱导的CTA。这些结果表明：（1）当用操作性药物辨别试验进行研究时，nAChR并非主要参与乙醇的辨别性刺激效应；（2）nAChR并非关键参与乙醇诱导的CTA。
