Ling J M, Koo I C, Kam K M, Cheng A F
Department of Microbiology, The Chinese University of Hong Kong, The Prince of Wales Hospital, Shatin, New Territories.
J Clin Microbiol. 1998 Jun;36(6):1693-9. doi: 10.1128/JCM.36.6.1693-1699.1998.
The incidence of salmonellosis has been increasing in Hong Kong since 1989. The most common Salmonella enterica serotype isolated in 1994 was S. enteritidis. The antimicrobial susceptibilities and molecular epidemiology of 275 S. enteritidis strains isolated in this locality between 1986 and 1996 were studied. Over 99% of the isolates were susceptible to 17 of the 19 antimicrobial agents tested. One isolate harbored an autotransferring plasmid that confers resistance to tetracycline and trimethoprim-sulfamethoxazole. Another isolate harbored a mobilizable plasmid that confers resistance to ampicillin and cephalothin. This isolate was found to produce a beta-lactamase with a pI of 5.2. A total of 264 isolates (96%) were found to harbor one to five plasmids, and the majority (254) harbored a 60-kb plasmid. Of these isolates, 94% contained identical 60-kb plasmids. Based on plasmid profiles, plasmid and chromosomal fingerprints, ribotypes, and randomly amplified polymorphic DNA (RAPD) patterns, 170 (62%) isolates were allocated to group 1b. About 90% of isolates had identical or similar DNA fingerprints, ribotypes, and RAPD patterns, suggesting that a predominant clone of S. enteritidis was circulating in Hong Kong during the period being studied.
自1989年以来，香港沙门氏菌病的发病率一直在上升。1994年分离出的最常见肠炎沙门氏菌血清型是肠炎沙门氏菌。对1986年至1996年期间在本地分离出的275株肠炎沙门氏菌菌株的抗菌药敏性和分子流行病学进行了研究。超过99%的分离株对所测试的19种抗菌药物中的17种敏感。一株分离株携带一种可自我转移的质粒，该质粒赋予对四环素和甲氧苄啶-磺胺甲恶唑的抗性。另一株分离株携带一种可移动质粒，该质粒赋予对氨苄青霉素和头孢菌素的抗性。该分离株被发现产生一种pI为5.2的β-内酰胺酶。总共264株分离株（96%）被发现携带一至五个质粒，大多数（254株）携带一个60 kb的质粒。在这些分离株中，有94%含有相同的60 kb质粒。根据质粒图谱、质粒和染色体指纹、核糖型以及随机扩增多态性DNA（RAPD）模式，170株（62%）分离株被归入1b组。约90%的分离株具有相同或相似的DNA指纹、核糖型和RAPD模式，这表明在研究期间，肠炎沙门氏菌的一个优势克隆在香港传播。
