Lerner L R, Gil M, Krisman C R
Instituto de Investigaciones Bioquímicas Luis F. Leloir Fundación Campomar, Buenos Aires, Argentina.
Cell Mol Biol (Noisy-le-grand). 1998 May;44(3):439-46.
Genesine, the naked autoglucosylating protein involved in glycogen biosynthesis, was partially purified from rat liver and some of its biochemical properties were characterized. Its activity was strongly activated by Mn2+ and two-pH optimums were obtained. UDP-14C-Glc was the preferred glucosyl donor substrate, but also UDP-14C-Xyl was. It was additionally found that more than one glucose was transferred to the protein or to that alpha1,4 glucan already linked to the protein from UDP-Glc. Glucose, maltose, xylose and UDP were inhibitors of genesine activity.
肌苷，一种参与糖原生物合成的裸自糖基化蛋白，从大鼠肝脏中部分纯化，并对其一些生化特性进行了表征。其活性被Mn2+强烈激活，并获得了两个最适pH值。UDP-14C-葡萄糖是首选的糖基供体底物，但UDP-14C-木糖也是。此外，还发现从UDP-葡萄糖转移到该蛋白或已与该蛋白相连的α1,4葡聚糖上的葡萄糖不止一个。葡萄糖、麦芽糖、木糖和UDP是肌苷活性的抑制剂。
