Berg S F, Dissen E, Westgaard I H, Fossum S
Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Norway.
Int Immunol. 1998 Apr;10(4):379-85. doi: 10.1093/intimm/10.4.379.
The gene for a rat NK lectin-like receptor (NKLLR), named NKR-P2, has been cloned and characterized. Sequence analysis shows that it represents the orthologue of human NKG2D and that the two molecules form a distinct NKLLR family, no more related to NKG2A/B, -C or -E than to other NKLLR families. Nkrp2 is a single-copy gene containing seven introns, mapping to the rat NK gene complex. Rat NKR-P2 differs from the human orthologue in that its cytoplasmic tail contains 13 additional amino acids, encoded by a separate exon. Splice variants lacking this exon were not detected in T cells or NK cells. NKR-P2 is strongly expressed by NK cells. In contrast to other NKLLR, it is also strongly expressed by resting thoracic duct CD4+ and CD8+ T cells, but not by thymocytes or other hemopoietic cells.
一种名为NKR-P2的大鼠自然杀伤（NK）凝集素样受体基因已被克隆并进行了特征分析。序列分析表明，它代表人类NKG2D的直系同源物，并且这两种分子形成了一个独特的NK凝集素样受体家族，与NKG2A/B、-C或-E的关系并不比与其他NK凝集素样受体家族的关系更密切。Nkrp2是一个单拷贝基因，包含7个内含子，定位于大鼠NK基因复合体。大鼠NKR-P2与人类直系同源物的不同之处在于，其胞质尾部含有另外13个氨基酸，由一个单独的外显子编码。在T细胞或NK细胞中未检测到缺少该外显子的剪接变体。NKR-P2在NK细胞中强烈表达。与其他NK凝集素样受体不同，它在静息胸导管CD4+和CD8+T细胞中也强烈表达，但在胸腺细胞或其他造血细胞中不表达。
