Ho E L, Heusel J W, Brown M G, Matsumoto K, Scalzo A A, Yokoyama W M
Howard Hughes Medical Institute, Division of Rheumatology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Proc Natl Acad Sci U S A. 1998 May 26;95(11):6320-5. doi: 10.1073/pnas.95.11.6320.
Natural killer (NK) cells express C-type lectin-like receptors, encoded in the NK gene complex, that interact with major histocompatibility complex class I and either inhibit or activate functional activity. Human NK cells express heterodimers consisting of CD94 and NKG2 family molecules, whereas murine NK cells express homodimers belonging to the Ly-49 family. The corresponding orthologues for other species, however, have not been described. In this report, we used probes derived from the expressed sequence tag database to clone C57BL/6-derived cDNAs homologous to human NKG2-D and CD94. Among normal tissues, murine NKG2-D and CD94 transcripts are highly expressed only in activated NK cells, including both Ly-49A+ and Ly-49A- subpopulations. Additionally, mNKG2-D is expressed in murine NK cell clones KY-1 and KY-2, whereas mCD94 expression is observed only in KY-1 cells but not KY-2. Last, we have finely mapped the physical location of the Cd94 (centromeric) and Nkg2d (telomeric) genes between Cd69 and the Ly49 cluster in the NK complex. Thus, these data indicate the expanding complexity of the NK complex and the corresponding repertoire of C-type lectin-like receptors on murine NK cells.
自然杀伤（NK）细胞表达由NK基因复合体编码的C型凝集素样受体，这些受体与主要组织相容性复合体I类相互作用，从而抑制或激活功能活性。人类NK细胞表达由CD94和NKG2家族分子组成的异二聚体，而小鼠NK细胞表达属于Ly-49家族的同二聚体。然而，尚未描述其他物种的相应直系同源物。在本报告中，我们使用来自表达序列标签数据库的探针克隆与人类NKG2-D和CD94同源的C57BL/6来源的cDNA。在正常组织中，小鼠NKG2-D和CD94转录本仅在活化的NK细胞中高表达，包括Ly-49A+和Ly-49A-亚群。此外，mNKG2-D在小鼠NK细胞克隆KY-1和KY-2中表达，而mCD94仅在KY-1细胞中表达，在KY-2细胞中未观察到。最后，我们精细地定位了NK复合体中Cd94（着丝粒）和Nkg2d（端粒）基因在Cd69和Ly49簇之间的物理位置。因此，这些数据表明NK复合体的复杂性不断增加，以及小鼠NK细胞上相应的C型凝集素样受体库。
