Shimoya K, Matsuzaki N, Sawai K, Saji F, Murata Y, Yasumoto K, Su S, Mukaida N, Matsushima K
Department of Obstetrics and Gynecology, Osaka University Medical School, Suita, Japan.
Mol Hum Reprod. 1998 Apr;4(4):393-400. doi: 10.1093/molehr/4.4.393.
A number of placental cytokines participate in the feto-maternal defence mechanism. Monocyte chemotactic and activating factor (MCAF) is one of these chemokines. We investigated the pattern of placental MCAF production, the localization of MCAF-producing cells in the placenta, and alterations in its expression in chorioamnionitis. The amounts of MCAF protein produced by the placenta increased during pregnancy irrespective of the presence of uterine contraction. MCAF mRNA was expressed at equivalent levels in the first and second trimester placenta, but at higher levels in the third trimester placenta. Chorioamnionitis in the third trimester placenta induced a 10-fold increase in MCAF protein production and a 3-fold increase in MCAF mRNA level. Immunohistochemical analysis of the placenta revealed the MCAF-producing cells to be trophoblasts. Stimulation of placental cells with lipopolysaccharide augmented MCAF production. These results indicate unique transcriptional and developmental regulation of MCAF mRNA and protein production during pregnancy and chorioamnionitis. Placental MCAF may be involved in the feto-maternal defence mechanism by activating feto-placental and maternal monocytes in chorioamnionitis.
多种胎盘细胞因子参与母胎防御机制。单核细胞趋化激活因子（MCAF）就是其中一种趋化因子。我们研究了胎盘MCAF的产生模式、胎盘内产生MCAF的细胞定位以及绒毛膜羊膜炎时其表达的变化。无论子宫收缩是否存在，胎盘产生的MCAF蛋白量在孕期都会增加。MCAF mRNA在孕早期和孕中期胎盘表达水平相当，但在孕晚期胎盘表达水平更高。孕晚期胎盘的绒毛膜羊膜炎导致MCAF蛋白产生增加10倍，MCAF mRNA水平增加3倍。胎盘的免疫组织化学分析显示产生MCAF的细胞为滋养层细胞。用脂多糖刺激胎盘细胞可增加MCAF的产生。这些结果表明孕期和绒毛膜羊膜炎期间MCAF mRNA和蛋白产生存在独特的转录和发育调控。胎盘MCAF可能通过在绒毛膜羊膜炎中激活胎儿 - 胎盘和母体单核细胞而参与母胎防御机制。
