Deleuran M, Buhl L, Ellingsen T, Harada A, Larsen C G, Matsushima K, Deleuran B
Department of Dermatology, University Hospital of Aarhus, Denmark.
J Dermatol Sci. 1996 Dec;13(3):228-36. doi: 10.1016/s0923-1811(96)00539-7.
The monocyte chemotactic protein-1 (MCAF) also termed MCP-1, a strong chemotactic factor towards monocytes, is produced by several cell types present in the skin. The in situ presence of MCAF/MCP-1 protein in the skin has, however, not yet been established. Using immunohistochemical techniques we have investigated the distribution of MCAF in skin from patients with different types of psoriasis and normal healthy volunteers. We report the novel finding that psoriasis has strong positive immunostaining for MCAF located to all the layers of the epidermis, except the stratum granulosum, in pustular, guttate and chronic plaque psoriasis. In the dermis, infiltrating cells in the perivascular aggregates and the blood vessels stained positive for MCAF. No significant differences were observed between the different subtypes of psoriasis except that strongly positive infiltrating cells were observed in the epidermal pustules in pustular psoriasis. In normals positive staining was observed in all the layers of the epidermis and in a few perivascular cells and blood vessels in the dermis. Where present in normal and diseased skin, eccrine ducts of sweat glands and sebaceous glands stained positive for MCAF. Arrector pili muscles were in all cases negative. These findings are consistent with a role for MCAF in attracting inflammatory cells, including monocytes, into the skin in psoriasis.
单核细胞趋化蛋白-1（MCAF），也称为MCP-1，是一种对单核细胞有强大趋化作用的因子，由皮肤中存在的几种细胞类型产生。然而，MCAF/MCP-1蛋白在皮肤中的原位存在尚未得到证实。我们使用免疫组织化学技术研究了不同类型银屑病患者和正常健康志愿者皮肤中MCAF的分布。我们报告了一项新发现，在脓疱型、点滴状和慢性斑块状银屑病中，除颗粒层外，银屑病表皮各层对MCAF均有强阳性免疫染色。在真皮中，血管周围聚集物中的浸润细胞和血管对MCAF染色呈阳性。除了在脓疱型银屑病的表皮脓疱中观察到强阳性浸润细胞外，不同亚型的银屑病之间未观察到显著差异。在正常人中，表皮各层以及真皮中的一些血管周围细胞和血管呈阳性染色。在正常皮肤和病变皮肤中，汗腺和皮脂腺的外分泌导管对MCAF染色呈阳性。立毛肌在所有情况下均为阴性。这些发现与MCAF在银屑病中吸引包括单核细胞在内的炎症细胞进入皮肤的作用一致。
