Czaplinski K, Ruiz-Echevarria M J, Paushkin S V, Han X, Weng Y, Perlick H A, Dietz H C, Ter-Avanesyan M D, Peltz S W
Department of Molecular Genetics and Microbiology, Robert Wood Johnson Medical School-UMDNJ, USA.
Genes Dev. 1998 Jun 1;12(11):1665-77. doi: 10.1101/gad.12.11.1665.
The nonsense-mediated mRNA decay pathway is an example of an evolutionarily conserved surveillance pathway that rids the cell of transcripts that contain nonsense mutations. The product of the UPF1 gene is a necessary component of the putative surveillance complex that recognizes and degrades aberrant mRNAs. Recent results indicate that the Upf1p also enhances translation termination at a nonsense codon. The results presented here demonstrate that the yeast and human forms of the Upf1p interact with both eukaryotic translation termination factors eRF1 and eRF3. Consistent with Upf1p interacting with the eRFs, the Upf1p is found in the prion-like aggregates that contain eRF1 and eRF3 observed in yeast [PSI+] strains. These results suggest that interaction of the Upf1p with the peptidyl release factors may be a key event in the assembly of the putative surveillance complex that enhances translation termination, monitors whether termination has occurred prematurely, and promotes degradation of aberrant transcripts.
无义介导的mRNA降解途径是一种进化上保守的监测途径的例子，该途径可清除细胞中含有无义突变的转录本。UPF1基因的产物是假定的监测复合物的必要组成部分，该复合物识别并降解异常mRNA。最近的结果表明，Upf1p还增强了无义密码子处的翻译终止。本文给出的结果表明，酵母和人类形式的Upf1p与真核翻译终止因子eRF1和eRF3都相互作用。与Upf1p与eRFs相互作用一致，在酵母[PSI+]菌株中观察到，Upf1p存在于含有eRF1和eRF3的朊病毒样聚集体中。这些结果表明，Upf1p与肽基释放因子的相互作用可能是假定的监测复合物组装中的关键事件，该复合物增强翻译终止、监测终止是否过早发生，并促进异常转录本的降解。
