乳酸乳球菌色氨酸操纵子的多重转录调控
Multiple transcriptional control of the Lactococcus lactis trp operon.
作者信息
Raya R, Bardowski J, Andersen P S, Ehrlich S D, Chopin A
机构信息
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
出版信息
J Bacteriol. 1998 Jun;180(12):3174-80. doi: 10.1128/JB.180.12.3174-3180.1998.
Abstract
The Lactococcus lactis trpEGDCFBA operon is preceded by a noncoding leader region. Transcriptional studies of the trp operon revealed three transcripts with respective sizes of 8 kb (encompassing the entire operon), 290 bases, and 160 bases (corresponding to parts of the leader region). These transcripts most likely result from initiation at the unique Ptrp promoter, transcription termination at either T1 (upstream of the trp operon) or T2 (downstream of the trp operon), and/or processing. Three parameters were shown to differentially affect the amount of these transcripts: (i) following tryptophan depletion, the amount of the 8-kb transcript increases 300- to 500-fold; (ii) depletion in any amino acid increased transcription initiation about fourfold; and (iii) upon entry into stationary phase the amount of the 8-kb transcript decreases abruptly. The tryptophan-dependent transcription control is exerted through transcription antitermination.
摘要
乳酸乳球菌trpEGDCFBA操纵子之前有一个非编码前导区。对trp操纵子的转录研究揭示了三种转录本，其大小分别为8 kb（包含整个操纵子）、290个碱基和160个碱基（对应于前导区的部分区域）。这些转录本很可能是由唯一的Ptrp启动子起始、在T1（trp操纵子上游）或T2（trp操纵子下游）处转录终止和/或加工产生的。有三个参数被证明对这些转录本的量有不同影响：（i）色氨酸耗尽后，8 kb转录本的量增加300至500倍；（ii）任何一种氨基酸的耗尽使转录起始增加约四倍；（iii）进入稳定期后，8 kb转录本的量突然减少。色氨酸依赖性转录控制是通过转录抗终止来实现的。
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