Abe A, Miyanohara A, Friedmann T
Department of Pediatrics, Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0634, USA.
J Virol. 1998 Jul;72(7):6159-63. doi: 10.1128/JVI.72.7.6159-6163.1998.
Exposure of Lipofectin-DNA complexes to the partially purified G glycoprotein of the vesicular stomatitis virus envelope (VSV-G) results in loss of serum-mediated inhibition and in enhanced efficiency of gene transfer. Sucrose density gradient sedimentation analysis indicated that the VSV-G associates physically with the DNA-lipid complex to produce a VSV-G liposome. The ability to incorporate surrogate viral or cellular envelope components such as VSV-G into liposomes may allow more-efficient and possibly targeted gene delivery by lipofection, both in vitro and in vivo.
将脂质体- DNA复合物暴露于水疱性口炎病毒包膜(VSV - G)的部分纯化的G糖蛋白中,会导致血清介导的抑制作用丧失,并提高基因转移效率。蔗糖密度梯度沉降分析表明,VSV - G与DNA - 脂质复合物物理结合,形成VSV - G脂质体。将替代病毒或细胞包膜成分(如VSV - G)掺入脂质体的能力,可能使脂质体转染在体外和体内实现更高效且可能是靶向性的基因递送。