卡波西肉瘤相关疱疹病毒DNA聚合酶及其持续合成因子的克隆与功能分析。
Cloning and functional analysis of Kaposi's sarcoma-associated herpesvirus DNA polymerase and its processivity factor.
作者信息
Lin K, Dai C Y, Ricciardi R P
机构信息
Department of Microbiology, School of Dental Medicine, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
出版信息
J Virol. 1998 Jul;72(7):6228-32. doi: 10.1128/JVI.72.7.6228-6232.1998.
Abstract
Kaposi's sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8, is a newly identified virus with tumorigenic potential. Here, we cloned and expressed the DNA polymerase (Pol-8) of KSHV and its processivity factor (PF-8). Pol-8 bound specifically to PF-8 in vitro. Moreover, the DNA synthesis activity of Pol-8 was shown in vitro to be strongly dependent on PF-8. Addition of PF-8 to Pol-8 allowed efficient synthesis of fully extended DNA products corresponding to the full-length M13 template (7,249 nucleotides), whereas Pol-8 alone could incorporate only several nucleotides. The specificity of PF-8 and Pol-8 for each other was demonstrated by their inability to be functionally replaced by the DNA polymerases and processivity factors of herpes simplex virus 1 and human herpesvirus 6.
摘要
卡波西肉瘤相关疱疹病毒（KSHV），即人类疱疹病毒8型，是一种新发现的具有致瘤潜力的病毒。在此，我们克隆并表达了KSHV的DNA聚合酶（Pol-8）及其持续合成因子（PF-8）。Pol-8在体外能与PF-8特异性结合。此外，体外实验表明Pol-8的DNA合成活性强烈依赖于PF-8。向Pol-8中添加PF-8能够高效合成与全长M13模板（7249个核苷酸）相对应的完全延伸的DNA产物，而单独的Pol-8只能掺入几个核苷酸。单纯疱疹病毒1型和人类疱疹病毒6型的DNA聚合酶及持续合成因子无法在功能上替代PF-8和Pol-8，这证明了PF-8和Pol-8彼此之间的特异性。
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