Russo J J, Bohenzky R A, Chien M C, Chen J, Yan M, Maddalena D, Parry J P, Peruzzi D, Edelman I S, Chang Y, Moore P S
Columbia Genome Center, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14862-7. doi: 10.1073/pnas.93.25.14862.
The genome of the Kaposi sarcoma-associated herpesvirus (KSHV or HHV8) was mapped with cosmid and phage genomic libraries from the BC-1 cell line. Its nucleotide sequence was determined except for a 3-kb region at the right end of the genome that was refractory to cloning. The BC-1 KSHV genome consists of a 140.5-kb-long unique coding region flanked by multiple G + C-rich 801-bp terminal repeat sequences. A genomic duplication that apparently arose in the parental tumor is present in this cell culture-derived strain. At least 81 ORFs, including 66 with homology to herpesvirus saimiri ORFs, and 5 internal repeat regions are present in the long unique region. The virus encodes homologs to complement-binding proteins, three cytokines (two macrophage inflammatory proteins and interleukin 6), dihydrofolate reductase, bcl-2, interferon regulatory factors, interleukin 8 receptor, neural cell adhesion molecule-like adhesin, and a D-type cyclin, as well as viral structural and metabolic proteins. Terminal repeat analysis of virus DNA from a KS lesion suggests a monoclonal expansion of KSHV in the KS tumor.
利用来自BC-1细胞系的黏粒和噬菌体基因组文库对卡波西肉瘤相关疱疹病毒（KSHV或HHV8）的基因组进行了定位。除了基因组右端一个难以克隆的3 kb区域外，其核苷酸序列已被确定。BC-1 KSHV基因组由一个140.5 kb长的独特编码区组成，两侧是多个富含G + C的801 bp末端重复序列。在这个源自细胞培养的毒株中存在一个显然在亲代肿瘤中出现的基因组重复。长独特区域中至少有81个开放阅读框，包括66个与猴疱疹病毒开放阅读框具有同源性的开放阅读框，以及5个内部重复区域。该病毒编码补体结合蛋白、三种细胞因子（两种巨噬细胞炎性蛋白和白细胞介素6）、二氢叶酸还原酶、bcl-2、干扰素调节因子、白细胞介素8受体、神经细胞黏附分子样黏附素和一种D型细胞周期蛋白的同源物，以及病毒结构蛋白和代谢蛋白。对卡波西肉瘤病变中病毒DNA的末端重复分析表明，KSHV在卡波西肉瘤肿瘤中呈单克隆扩增。
