Kishko Ia H, Vasylenko M I, Pidhors'kyĭ V S, Kovalenko E O
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Mikrobiol Z. 1997 Nov-Dec;59(6):20-6.
It has been demonstrated experimentally that lectin of Bacillus subtilis sp. in comparison with generally accepted Con A, PHA and lectin of "gold rain" grass--Laburnum anagyroides M e d i k in trials on white mice of CBA line gave in 4 hours of induction maximal titers of gamma-IFN in blood serum of animals--153.6 +/- 17.0 IU/ml. Practically identical titers had been obtained after induction by lectin "gold rain", some lower--after Con A and PHA. At swine gamma-IFN synthesis optimal density of cell suspension must contain 2.5 + 10(7) immunocytes in 1 ml, owing to which it is possible to obtain the titer equal 1 : 2150. Materials with using of bacterial lectins at various degree of purification had shown that maximal titers in blood serum of mongrel white mice were registered at administration to animals of non-purified lectin, 4 times lower--at using of half-purified and purified lectins. Data of these trials in vivo were confirmed by materials of gamma-IFN induction by immunocytes of swine, cattle and even man.
实验证明，在对CBA系小白鼠进行的试验中，枯草芽孢杆菌的凝集素与公认的刀豆球蛋白A、植物血凝素以及“金雨”草（披针叶黄花木）的凝集素相比，在诱导4小时后，动物血清中γ-干扰素的滴度达到最大值——153.6±17.0 IU/ml。用“金雨”凝集素诱导后得到的滴度实际上相同，刀豆球蛋白A和植物血凝素诱导后的滴度略低。对于猪γ-干扰素的合成，细胞悬液的最佳密度必须是每毫升含有2.5×10⁷个免疫细胞，由此可获得的滴度等于1:2150。使用不同纯化程度的细菌凝集素的材料表明，给杂种小白鼠注射未纯化的凝集素时，血清中的滴度最高，使用半纯化和纯化凝集素时的滴度低4倍。猪、牛甚至人的免疫细胞诱导γ-干扰素的材料证实了这些体内试验的数据。
