Veneziale C M, Deering L C
Andrologia. 1976;8(2):73-82. doi: 10.1111/j.1439-0272.1976.tb02112.x.
Luminal contents of guinea pig seminal vesicle were extracted with 0.154 M NaCl and the soluble protein fraction was studied by DEAE-cellulose and Sephadex G-100 column chromatography; anionic, cationic, and SDS-polyarcylamide disc gel electrophoresis; isoelectric focusing; molecular weight determinations by SDS-polyacrylamide disc gel electrophoresis; analysis for neutral carbohydrate; sucrose density-gradient centrifugation for determination of sedimentation coefficients; and amino acid analyses. Four protein fractions (referred to as proteins 1, 2, 3, and 4 in order to elution) were obtained by DEAE-cellulose column chromatography. Proteins 1, 2, and 4 gave single major bands in multiple disc gel electrophoretic systems. Protein 3 gave one major band and one minor component believed to be a fragment of a primary vesicular protein. Protein 1 is very basic and probably represents the clotting protein of guinea pig semen. The three nonclotting proteins could not be detected in serum and are probably intrinsic to seminal vesicle epithelium. All four proteins were found in animals weighing 700 g or more; only two of the three nonclotting proteins were found in more than half of the younger animals. This makes it even more likely that one or more of the nonclotting proteinc could serve as a useful gene marker in studies of androgen mechanisms.
用0.154M氯化钠提取豚鼠精囊的腔内物质，并用DEAE - 纤维素和葡聚糖G - 100柱色谱法研究其可溶性蛋白部分；进行阴离子、阳离子和SDS - 聚丙烯酰胺圆盘凝胶电泳；等电聚焦；通过SDS - 聚丙烯酰胺圆盘凝胶电泳测定分子量；分析中性碳水化合物；用蔗糖密度梯度离心法测定沉降系数；以及氨基酸分析。通过DEAE - 纤维素柱色谱法获得了四个蛋白组分（按洗脱顺序称为蛋白1、2、3和4）。在多个圆盘凝胶电泳系统中，蛋白1、2和4呈现单一主带。蛋白3呈现一条主带和一个次要成分，推测该次要成分是一种初级囊泡蛋白的片段。蛋白1碱性很强，可能代表豚鼠精液的凝固蛋白。在血清中未检测到这三种非凝固蛋白，它们可能是精囊上皮所特有的。在体重700克或以上的动物中发现了所有四种蛋白；在超过半数的较年轻动物中只发现了三种非凝固蛋白中的两种。这使得一种或多种非凝固蛋白更有可能在雄激素机制的研究中作为有用的基因标记物。
