Kimura M, Furukawa T, Abe T, Yatsuoka T, Youssef E M, Yokoyama T, Ouyang H, Ohnishi Y, Sunamura M, Kobari M, Matsuno S, Horii A
Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan.
Cancer Res. 1998 Jun 1;58(11):2456-60.
Using the method of microsatellite analysis, we studied 40 tissues with pancreatic ductal adenocarcinoma and identified two commonly deleted regions on the long arm of chromosome 12. One (region A) was found between D12S81 and D12S1719 at 12q21 at a frequency of 67.5%, and the other (region B) was located between D12S360 and D12S78 at 12q22-q23.1 at a frequency of 60%; the latter was reported previously (M. Kimura, et al. Genes Chromosomes Cancer, 17: 88-93, 1996). The results of microsatellite analyses were verified by fluorescence in situ hybridization. We further analyzed 19 pancreatic cancer cell lines by fluorescence in situ hybridization and found that 10 of them showed allelic loss at D12S81 and 6 showed allelic loss at D12S360. Yeast artificial chromosome contigs were constructed to cover the deleted regions. Region B was completely covered by a 650-kb yeast artificial chromosome clone. The frequently deleted regions in chromosome 12q in pancreatic cancer that were identified here may provide new avenues for isolating novel tumor suppressor genes.
我们采用微卫星分析方法，对40例胰腺导管腺癌组织进行了研究，确定了12号染色体长臂上的两个常见缺失区域。一个区域（A区）位于12q21的D12S81和D12S1719之间，缺失频率为67.5%；另一个区域（B区）位于12q22 - q23.1的D12S360和D12S78之间，缺失频率为60%；后者曾有报道（M. Kimura等人，《基因与染色体癌症》，17: 88 - 93, 1996年）。微卫星分析结果通过荧光原位杂交得以验证。我们进一步通过荧光原位杂交对19个胰腺癌细胞系进行分析，发现其中10个在D12S81处出现等位基因缺失，6个在D12S360处出现等位基因缺失。构建酵母人工染色体重叠群以覆盖缺失区域。B区被一个650 kb的酵母人工染色体克隆完全覆盖。此处确定的胰腺癌12q染色体上的常见缺失区域可能为分离新的肿瘤抑制基因提供新途径。
