人类子宫内膜癌10号染色体q25 - q26区域常见等位基因缺失的790千碱基区域的鉴定。

Identification of a 790-kilobase region of common allelic loss in chromosome 10q25-q26 in human endometrial cancer.

作者信息

Nagase S, Yamakawa H, Sato S, Yajima A, Horii A

机构信息

Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan.

出版信息

Cancer Res. 1997 May 1;57(9):1630-3.

PMID:9134997

Abstract

We previously identified two commonly deleted regions on chromosome bands 10q25-q26 in endometrial cancer: an 8-cM region and a 12-cM region. In the present study, we further studied the 8-cM region with 83 endometrial cancer specimens and 14 microsatellite markers and narrowed it down to a 1-cM region between D10S587 and D10S1723. This result was confirmed by two-color fluorescence in situ hybridization analysis. An association between histopathologically lower grade tumor and allelic loss (P = 0.03 by Fisher's exact test) was also observed. We also constructed a yeast artificial chromosome (YAC) contig and found that one YAC clone, which was 790 kb in size, harbored the whole 1-cM region of common allelic loss.

摘要

我们之前在子宫内膜癌的10q25 - q26染色体带上鉴定出两个常见的缺失区域：一个8厘摩（cM）区域和一个12厘摩区域。在本研究中，我们用83个子宫内膜癌标本和14个微卫星标记进一步研究了这个8厘摩区域，并将其缩小到D10S587和D10S1723之间的一个1厘摩区域。该结果通过双色荧光原位杂交分析得到证实。还观察到组织病理学分级较低的肿瘤与等位基因缺失之间存在关联（Fisher精确检验，P = 0.03）。我们还构建了一个酵母人工染色体（YAC）重叠群，发现一个大小为790 kb的YAC克隆包含了整个1厘摩常见等位基因缺失区域。