用于免疫细胞化学分析的空气干燥细胞学标本上热不稳定抗原的制备。
Preparation of heat-labile antigens on air-dried cytologic specimens for immunocytochemical analysis.
作者信息
Baba N, Kawabata H, Kanno T, Ueno T, Makuuchi M, Morishita Y, Mori S, Sasaki J, Soejima K
机构信息
2nd Department of Surgery, Faculty of Medicine, University of Tokyo, Japan.
出版信息
Acta Cytol. 1998 May-Jun;42(3):707-15. doi: 10.1159/000331831.
OBJECTIVE
To demonstrate the efficacy of Zamboni's solution and storage medium for immunocytochemistry of Ki-67, p53 tumor suppressor gene product and CD3 on long-preserved, air-dried cytologic specimens.
STUDY DESIGN
Air-dried imprint cytologic specimens obtained from 13 lymphatic tissues and 13 colorectal cancers were fixed with Zamboni's solution and stored for various periods in storage medium including 50% glycerol at -10 to -20 degree C. Mouse monoclonal antibody PC, PAb 1801 and anti-Leu-4 were used for immunostaining.
RESULTS
Immunocytochemical identification of Ki-67, p53 and CD3 was possible on specimens stored for 36, 32 and 16 weeks, respectively.
CONCLUSION
Our method, using Zamboni's solution and storage medium, is feasible for immunocytochemistry on long-preserved, air-dried cytologic specimens using the three antibodies were employed.
目的
证明赞博尼固定液及储存介质在对长期保存的风干细胞学标本进行Ki-67、p53肿瘤抑制基因产物和CD3免疫细胞化学检测中的有效性。
研究设计
从13例淋巴组织和13例结肠直肠癌获取的风干印片细胞学标本，用赞博尼固定液固定，并在包括-10至-20℃的50%甘油的储存介质中储存不同时间。使用小鼠单克隆抗体PC、PAb 1801和抗-Leu-4进行免疫染色。
结果
分别在储存36周、32周和16周的标本上进行Ki-67、p53和CD3的免疫细胞化学鉴定是可行的。
结论
我们使用赞博尼固定液和储存介质的方法，对于使用上述三种抗体对长期保存的风干细胞学标本进行免疫细胞化学检测是可行的。
Zotero 插件