Engin C
Clinic of Plastic Surgery, Ankara Railway Hospital, Turkey.
Plast Reconstr Surg. 1998 Jun;101(7):1887-90. doi: 10.1097/00006534-199806000-00017.
The aim of the present study was to observe the effect of calcitonin gene-related peptide (CGRP) on wound contraction in both denervated and normal areas. A total of 100 Wistar rats, each of which had a 2 x 2 cm full-thickness skin defect on the back, were divided into two main control groups and six corresponding experimental groups in which 10 x 10(-9) M and 10 x 10(-12) M synthetic rat CGRP were given intraperitoneally and intradermally. Contraction was assessed weekly with planimetry, and mean surface areas (mm2 +/- SD) of related groups were compared. Complete closure took 4 weeks for the normal control group and 8 weeks for the denervated control group (p < 0.05). The 10 x 10(-12) M CGRP with both types of application showed a decrease in the length of time for complete closure to 3 weeks in the normal experimental groups (p < 0.05), but complete closure still took 4 weeks in normal groups in which 10 x 10(-9) M CGRP was given (p < 0.05). Any dosage of CGRP given intraperitoneally showed no change in the closure period in the denervated experimental groups (p > 0.05). CGRP showed a trophic effect on healing by an increased rate of contraction in the rat model. However, the neural supply to the wound area seemed to be intact because of the necessity of axonal transfer of CGRP.
本研究的目的是观察降钙素基因相关肽（CGRP）对去神经支配区域和正常区域伤口收缩的影响。将100只背部有2×2cm全层皮肤缺损的Wistar大鼠分为两个主要对照组和六个相应的实验组，分别腹腔内和皮内注射10×10⁻⁹M和10×10⁻¹²M的合成大鼠CGRP。每周用面积测量法评估伤口收缩情况，并比较相关组的平均表面积（mm²±标准差）。正常对照组伤口完全闭合需4周，去神经支配对照组需8周（p<0.05）。两种给药方式的10×10⁻¹²M CGRP使正常实验组伤口完全闭合时间缩短至3周（p<0.05），但给予10×10⁻⁹M CGRP的正常组伤口完全闭合仍需4周（p<0.05）。腹腔内注射任何剂量的CGRP，去神经支配实验组的伤口闭合时间均无变化（p>0.05）。在大鼠模型中，CGRP通过提高收缩率对愈合表现出营养作用。然而，由于CGRP需要轴突转运，伤口区域的神经供应似乎是完整的。
