Engin C, Demirkan F, Ayhan S, Atabay K, Baran N K
Division of Plastic Surgery, Ankara Railway Hospital, Turkey.
Plast Reconstr Surg. 1996 Nov;98(6):1063-7. doi: 10.1097/00006534-199611000-00021.
Neuronal supply in soft tissues may be an important part of cutaneous wound healing. In order to observe the effect of denervation on wound contraction, rectangular full-thickness skin defects were created on the dorsum of two groups of Wistar rats. In the experimental group (n = 20), spinal nerves corresponding to the area of the open wound (T11 to L2) were isolated and divided bilaterally. In the control group (n = 20), the same pairs of spinal nerves were dissected but left intact. Limits of denervation were verified by the pinprick test. Wound healing, which is primarily in the form of wound contraction in this model, was evaluated by tracing wound margins onto millimetric paper weekly. Wound contraction was delayed significantly in the experimental group (p < 0.05) at all follow-up periods when compared with the controls. Loss of neuropeptide secretion from the nerve endings in denervated tissues may be responsible for the retarded wound contraction, since neuropeptides are thought to exert trophic effects on skin wound healing.
软组织中的神经供应可能是皮肤伤口愈合的重要组成部分。为了观察去神经支配对伤口收缩的影响，在两组Wistar大鼠的背部制作了矩形全层皮肤缺损。在实验组（n = 20）中，分离并双侧切断与开放性伤口区域相对应的脊神经（T11至L2）。在对照组（n = 20）中，解剖相同的脊神经对但保持完整。通过针刺试验验证去神经支配的范围。在该模型中，伤口愈合主要以伤口收缩的形式进行，每周通过将伤口边缘描绘在毫米纸上进行评估。与对照组相比，实验组在所有随访期的伤口收缩均显著延迟（p < 0.05）。去神经支配组织中神经末梢神经肽分泌的丧失可能是伤口收缩延迟的原因，因为神经肽被认为对皮肤伤口愈合具有营养作用。
