Walderich B, Bernauer S, Renner M, Knobloch J, Burchard G D
Institut für Tropenmedizin, Tübingen, Germany.
Trop Med Int Health. 1998 May;3(5):385-90. doi: 10.1046/j.1365-3156.1998.00241.x.
Blastocystis hominis isolates from asymptomatic carriers and symptomatic patients were cultured in vitro, purified from the co-cultivated bacterial flora and tested for cytopathic effects on monolayers of Chinese Hamster Ovary (CHO) cells and Adeno Carcinoma HT29 cells. In the case of the CHO cells, living B. hominis cells and B. hominis cell lysates were able to cause significant cytopathic effects, which were dependent on the concentration of cells employed. Destruction of the cell monolayers was observed to the same extent with patient isolates derived from healthy or symptomatic B. hominis carriers. HT29 cells were less susceptible: B. hominis cells and cell lysates caused only minor effects which were not statistically significant. Culture filtrates of B. hominis exhibited cytopathic potential on CHO and HT29 cells; however, the control which consisted of filtrates from Robinson's cultures in which B. hominis failed to grow showed similar effects, too. Therefore the culture supernatants could not be proven to produce a specific cytopathic effect on CHO and HT29 cells.
从无症状携带者和有症状患者中分离出的人芽囊原虫菌株在体外进行培养，从共培养的细菌菌群中纯化出来，并检测其对中国仓鼠卵巢（CHO）细胞单层和腺癌HT29细胞的细胞病变效应。对于CHO细胞，活的人芽囊原虫细胞和人芽囊原虫细胞裂解物能够引起显著的细胞病变效应，这取决于所用细胞的浓度。从健康或有症状的人芽囊原虫携带者分离出的患者菌株对细胞单层的破坏程度相同。HT29细胞较不敏感：人芽囊原虫细胞和细胞裂解物仅产生轻微影响，无统计学意义。人芽囊原虫的培养滤液对CHO和HT29细胞表现出细胞病变潜能；然而，由人芽囊原虫未能生长的罗宾逊培养基滤液组成的对照也显示出类似的效应。因此，无法证明培养上清液对CHO和HT29细胞产生特异性细胞病变效应。
