使用连续培养生物膜系统研究阴道加德纳菌和嗜酸乳杆菌的抗菌药敏性。
Use of a continuous-culture biofilm system to study the antimicrobial susceptibilities of Gardnerella vaginalis and Lactobacillus acidophilus.
作者信息
Muli F, Struthers J K
机构信息
Department of Medical Microbiology, Manchester Royal Infirmary, United Kingdom.
出版信息
Antimicrob Agents Chemother. 1998 Jun;42(6):1428-32. doi: 10.1128/AAC.42.6.1428.
Abstract
Gardnerella vaginalis and Lactobacillus acidophilus have been shown to grow to high titers in a simple biofilm system. This system was used in the present investigation to compare the biofilm-eradicating concentrations (BECs) of amoxicillin, clindamycin, erythromycin, and metronidazole to standard tube MIC and minimum bactericidal concentration (MBC) results. With the lactobacillus, the BEC/tube MBC ratio was at least 16:1, while for G. vaginalis the ratio varied from 2:1 to 512:1. The simple continuous-culture system used in the present investigation is ideal for investigating the BEC for bacteria involved in complex ecological situations such as bacterial vaginosis and may be useful for the identification of the most effective and selective antibiotic therapy.
摘要
已证明阴道加德纳菌和嗜酸乳杆菌在一个简单的生物膜系统中能生长至高滴度。在本研究中使用该系统将阿莫西林、克林霉素、红霉素和甲硝唑的生物膜消除浓度（BEC）与标准试管最低抑菌浓度（MIC）和最低杀菌浓度（MBC）结果进行比较。对于乳酸杆菌，BEC/试管MBC比值至少为16:1，而对于阴道加德纳菌，该比值在2:1至512:1之间变化。本研究中使用的简单连续培养系统非常适合研究参与诸如细菌性阴道病等复杂生态情况的细菌的BEC，并且可能有助于确定最有效和最具选择性的抗生素治疗方法。
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本文引用的文献
1
A simple in vitro model for growth control of bacterial biofilms.
J Appl Bacteriol. 1995 Jul;79(1):87-93. doi: 10.1111/j.1365-2672.1995.tb03128.x.
2
The growth of Gardnerella vaginalis and Lactobacillus acidophilus in Sorbarod biofilms.
J Med Microbiol. 1998 May;47(5):401-5. doi: 10.1099/00222615-47-5-401.
3
The use of Sorbarod biofilms to study the antimicrobial susceptibility of a strain of Streptococcus pneumoniae.使用Sorbarod生物膜研究一株肺炎链球菌的抗菌药敏性。
J Antimicrob Chemother. 1997 Oct;40(4):601-2. doi: 10.1093/jac/40.4.601.
4
Tackling bacterial vaginosis and HIV in developing countries.应对发展中国家的细菌性阴道病和艾滋病病毒
Lancet. 1997 Aug 23;350(9077):530-1. doi: 10.1016/S0140-6736(97)22034-9.
5
Bacterial biofilms: resistance to antimicrobial agents.细菌生物膜：对抗菌剂的耐药性
J Antimicrob Chemother. 1996 Jun;37(6):1047-50. doi: 10.1093/jac/37.6.1047.
6
Bacterial vaginosis in pregnancy: distribution of bacterial species in different gram-stain categories of the vaginal flora.妊娠期细菌性阴道病：阴道菌群不同革兰氏染色类别中细菌种类的分布
J Med Microbiol. 1996 Aug;45(2):120-6. doi: 10.1099/00222615-45-2-120.
7
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8
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10
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Clin Microbiol Rev. 1991 Oct;4(4):485-502. doi: 10.1128/CMR.4.4.485.
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